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茶皂素杀线虫活性测定分析
引用本文:吴慧平,宛晓春,侯如燕,徐晓莉.茶皂素杀线虫活性测定分析[J].植物病理学报,2007,37(5):553-555.
作者姓名:吴慧平  宛晓春  侯如燕  徐晓莉
作者单位:1 安徽农业大学农业部/教育部茶叶生物化学与生物技术重点实验室 合肥 230036;2 安徽农业大学植物保护学院 合肥 230036
摘    要: 茶皂素(Tea saponin, TS)是由各类茶籽(油茶Thea sinesis或茶Camellia sasauqua)饼粕中提取出来的五环三萜类化合物,具表面活性、生理活性和鱼毒活性以及溶血性等。近10年来,国内有关TS杀虫抑菌活性有较多的研究报道,但未见杀线虫活性方面的报道。

关 键 词:杀线虫活性  测定分析  茶皂素  Camellia  三萜类化合物  表面活性  生理活性  抑菌活性
文章编号:0412-0914(2007)05-0553-03
收稿时间:2006-10-12
修稿时间:2006-10-12

Bioassay of nematicidal activity of Tea saponin
WU Hui-ping, WAN Xiao-chun, HOU Ru-yan , XU Xiao-li.Bioassay of nematicidal activity of Tea saponin[J].Acta Phytopathologica Sinica,2007,37(5):553-555.
Authors:WU Hui-ping  WAN Xiao-chun  HOU Ru-yan  XU Xiao-li
Institution:1 Key Laboratory of Tea Biochemistry & Biotechnology of Ministry of Agriculture, Anhui Agricultural University, Hefei 230036, China;2 School of Plant Protection, AAU, Hefei 230036, China
Abstract:In order to determine the nematicidal activity of Tea saponin(TS), the oil tea cakes were processed through degreasing, dealcholizing, and extracting with n-butyl alcohol, the crude powder of 84.6% TS was obtained. The TS powder was evaluated against 4 plant nematodes and 1 soil nematode. The results indicated that TS exhibited modest nematicidal activity against the 4 plant nematodes, but exhibited no nematicidal activity against Rhbiditis sp.. The pot experiments showed that infective inhibition of TS against Hrischamanniells oryzae is 71.1%, galls on Trichosanthes kirilowii caused by Meloidogyne incognita was reduced by 88.9% when the bare-root-tip was placed in TS solution for 3 h.
Keywords:Tea saponin  plant parasitic nematode  nematicidal activity
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