| 洋葱伯克霍尔德氏菌株Lyc2的鉴定及对棉苗的防病促生作用 |
| |
| 引用本文： | 于晓庆,郗丽君,刘永光,李光伟,兰玉菲,竺晓平,李向东.洋葱伯克霍尔德氏菌株Lyc2的鉴定及对棉苗的防病促生作用[J].植物病理学报,2007,37(4):426-432. |
| |
| 作者姓名： | 于晓庆 郗丽君 刘永光 李光伟 兰玉菲 竺晓平 李向东 |
| |
| 作者单位： | 1 山东农业大学植物保护学院植物病理系, 泰安 271018;2 山东省作物生物学重点实验室, 泰安 271018;3 青州市农业局, 青州 262500 |
| |
| 基金项目： | 山东省优秀中青年科学家科研奖励基金;山东农业大学校科研和教改项目 |
| |
| 摘 要： | 从烟草根际土壤中分离到一株细菌菌株Lyc2,平皿对峙培养显示该菌可显著抑制多种植物病原真菌菌丝体的生长。温室盆栽试验表明,Lyc2对棉花苗期立枯病的防治效果达到48.8%;水培棉花苗试验表明,Lyc2能显著增加棉苗的鲜重和干重,但对株高的影响不显著。该菌经形态、生理生化试验测定及16S rDNA和ITS序列分析,初步确定为洋葱伯克霍尔德氏菌(Burkholderia cepacia);进一步通过种特异的recA基因序列分析,证明Lyc2菌株属于B. cepacia复合物中基因型IX,B.pyrrocinia。
|
| 关 键 词： | 植物根际促生菌 洋葱伯克霍尔德氏菌 16S rDNA 16S-23S rDNA转录单元内间隔区序列(ITS) recA基因 |
| 文章编号： | 0412-0914（2007）04-0426-07 |
| 修稿时间： | 2006-05-09 |
Physio-biochemical characterization and molecular identification of Burkholderia cepacia isolate Lyc2 as a PGPR to cotton seedlings |
| |
| YU Xiao-qing,XI Li-jun,LIU Yong-guang,LI Guang-wei,LAN Yu-fei,ZHU Xiao-ping,LI Xiang-dong.Physio-biochemical characterization and molecular identification of Burkholderia cepacia isolate Lyc2 as a PGPR to cotton seedlings[J].Acta Phytopathologica Sinica,2007,37(4):426-432. |
| |
| Authors: | YU Xiao-qing XI Li-jun LIU Yong-guang LI Guang-wei LAN Yu-fei ZHU Xiao-ping LI Xiang-dong |
| |
| Institution: | 1 Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Taian 271018, China;2 Key Laboratory of Crop Biology in Shandong Province, Taian 271018, China;3 Qingzhou Agricultural Bureau, Qingzhou 262500, China |
| |
| Abstract: | A bacterial strain,Lyc2,was isolated from tobacco rhizosphere and showed remarkable inhibition to the mycelial growth of multiple pathogenic fungi in dual culture.In greenhouse,strain Lyc2 showed 48.8% efficiency to control cotton seedling damping-off.In solution culture experiment,compared with control,Lyc2 significantly increased the fresh weight and dry weight of cotton seedlings.The morphological,physiological and biochemical characteristics of Lyc2 were similar to those of Burkholderia cepacia.Homology analysis of 16S rDNA and ITS sequences showed that Lyc2 was closely related to B.cepacia.Further results of recA gene sequence comparison showed that Lyc2 belonged to B.pyrrocinia,genomovar IX of B.cepacia complex. |
| |
| Keywords: | 16S rDNA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《植物病理学报》浏览原始摘要信息 |
| 点击此处可从《植物病理学报》下载免费的PDF全文 |
|
