| 多粘芽孢杆菌JW-725抗菌活性物质及其发酵条件的初步研究 |
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| 引用本文： | 赵德立,曾林子,李晖,陈金瑞,刘成君.多粘芽孢杆菌JW-725抗菌活性物质及其发酵条件的初步研究[J].植物保护,2006,32(1):47-50. |
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| 作者姓名： | 赵德立 曾林子 李晖 陈金瑞 刘成君 |
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| 作者单位： | 1. 四川大学生命科学学院,成都,610064
2. 西藏高原生物研究所,拉萨,850001 |
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| 摘 要： | 多粘芽孢杆菌JW-725对柑橘青霉具有很强的拮抗作用。通过5因素4水平正交试验明确,应用LB培养基培养,初始pH 9.0,30℃,通气量100 mL,接种量为10%,140 r/min振荡培养72 h时的发酵液具有最佳抗菌活性。该菌胞外抗菌物质可用乙酸乙酯单一溶剂提取。
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| 关 键 词： | 生物工程 多粘芽孢杆菌 抗真菌活性物质 发酵条件 |
| 收稿时间： | 05 15 2005 12:00AM |
| 修稿时间： | 2005-05-152005-08-23 |
Fermentation of Bacillus polymyxa JW-725 and preliminary extraction of the antifungal substances |
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| Zhao Deli;Zeng Linzi;Li Hui;Chen Jinrui Liu Chengjun;.Fermentation of Bacillus polymyxa JW-725 and preliminary extraction of the antifungal substances[J].Plant Protection,2006,32(1):47-50. |
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| Authors: | Zhao Deli;Zeng Linzi;Li Hui;Chen Jinrui Liu Chengjun; |
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| Institution: | 1. Collegeof Life Science,Sichuan University,Chengdu 610064,China;2. Tibet Institute of Tableland Biology,Lasa 850001,China |
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| Abstract: | Bacillus polymyxa JW-725 has a significant antifungal effect on Penicillium italicum.The five-factor and 4-grade orthogonal experiments found that the fermentative liquid had the strongest antifungal effect,with the following fermentative conditions: LB media with initial pH9.0,30℃,72 h,10% inoculum and 100 mL culture in a 500 mL bottle.Further studies showed that the strain secreted an extracellular antifungal product that could be extracted preliminarily by ethyl acetate. |
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| Keywords: | bioengineering Bacillus polymyxa antifungal substance fermentation conditions |
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