| 基于6-BA／NAA的黑树莓不定芽再生的研究 |
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| 引用本文： | 哈玲津,马颖,杨静慧.基于6-BA／NAA的黑树莓不定芽再生的研究[J].天津农学院学报,2009,16(2):15-16,30. |
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| 作者姓名： | 哈玲津 马颖 杨静慧 |
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| 作者单位： | 1. 天津农学院农业分析实验室,天津,300384
2. 天津农学院园艺系,天津,300384 |
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| 基金项目： | 天津市科委科技支撑重点项目，天津市农委资助项目 |
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| 摘 要： | 为了快速繁殖优良种黑树莓，进行了不同激素、不同外植体的组织培养和再生研究。结果显示：在不同6．BA浓度培养下，均为茎段的不定芽的再生率最高，叶柄次之，叶片最低。并且，随着培养基6．BA浓度的增加，各外植体的芽分化率具有相同的变化趋势，均呈抛物线型变化。各外植体均在4．0mg．L～6-BA和0．1mg．L-1 NAA时芽的分化率最高，其中黑树莓茎段的不定芽的再生率高达87．50％。
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| 关 键 词： | 黑树莓 外植体：组织培养 离体再生 |
Concentration of 6-BA/NAA Regeneration in Black Raspberry |
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| HA Ling-jin,MA Ying,YANG Jing-hui.Concentration of 6-BA/NAA Regeneration in Black Raspberry[J].Journal of Tianjin Agricultural College,2009,16(2):15-16,30. |
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| Authors: | HA Ling-jin MA Ying YANG Jing-hui |
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| Institution: | 1. Tianjin Agricultural University, a. Laboratory of Agriculture Analysis, b. Department of Horticulture, Tianjin 300384, China) |
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| Abstract: | In order to propagate the black raspberry rapidly, the tissue culture and regeneration of black raspberry was studied with different concentrations of 6-BA and NAA. The results show that the bud regeneration percent of stem segments was the highest, the next was the leave stalks, and the lowest was the leaves under different 6-BA concentration. Besides, the change trend of bud regeneration rate of different explants was the same, and appeared parabola. All kinds of explants appeared the highest bud regeneration rate under 4.0 mg·L-1 6-BA and 0.1 mg·L-1 NAA, in which the bud regeneration rate was 87.50% by stem segments. |
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| Keywords: | black raspberry explants tissue culture regeneration in vitro |
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