Putative virulence-related genes in Vibrio anguillarum identified by random genome sequencing |
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| Authors: | Rodkhum C Hirono I Stork M Di Lorenzo M Crosa J H Aoki T |
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| Institution: | Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, Japan. |
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| Abstract: | The genome of Vibrio anguillarum strain H775-3 was partially determined by a random sequencing procedure. A total of 2,300 clones, 2,100 from a plasmid library and 200 from a cosmid library, were sequenced and subjected to homology search by the BLAST algorithm. The total length of the sequenced clones is 1.5 Mbp. The nucleotide sequences were classified into 17 broad functional categories. Forty putative virulence-related genes were identified, 36 of which are novel in V. anguillarum, including a repeat in toxin gene cluster, haemolysin genes, enterobactin gene, protease genes, lipopolysaccharide biosynthesis genes, capsule biosynthesis gene, flagellar genes and pilus genes. |
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| Keywords: | colonization factors exotoxins lipopolysaccharide random genome sequencing Vibrio anguillarum virulence |
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