| O型口蹄疫病毒间接免疫荧光检测方法的建立 |
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| 引用本文： | 蔡扩军,乔 军,孟庆玲,陈创夫,马铈委,黄 炯,魏 婕.O型口蹄疫病毒间接免疫荧光检测方法的建立[J].西北农业学报,2012,21(6):13-16. |
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| 作者姓名： | 蔡扩军 乔 军 孟庆玲 陈创夫 马铈委 黄 炯 魏 婕 |
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| 作者单位： | 1. 石河子大学动物科技学院,新疆石河子,832003
2. 新疆畜牧科学院兽医研究所,乌鲁木齐,830000 |
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| 摘 要： | 为建立猪O型口蹄疫病毒(FMDV)间接免疫荧光(IFA)检测方法,以猪抗O型口蹄疫病毒阳性血清为一抗、异硫氰酸荧光素(FITC)标记的SPA蛋白(葡萄球菌蛋白A)为二抗,通过反应条件的优化,建立检测方法。结果表明,IFA最佳工作条件为,一抗最适稀释度为1∶50,FITC标记的二抗的最适稀释度为1∶800,最适孵育时间均为1h。特异性试验表明,用建立的IFA检测方法只能检测O型FMDV,而A型、AsiaⅠFMDV型和猪水疱病病毒(SVDV)检测结果均为阴性。建立的检测O型FMDV抗原的IFA检测方法具有特异、敏感、简便、快速等优点,可用于FMDV感染的实验室诊断及其在感染机体中的定位和动态分布研究。
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| 关 键 词： | O型口蹄疫病毒 间接免疫荧光 |
Establishment of Indirect Immunofluorescence Assay for Detection of Foot and Mouth Disease Virus Serotype O |
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| CAI Kuojun,QIAO Jun,MENG Qingling,CHEN Chuangfu,MA Shiwei,HUANG Jiong and WEI Jie.Establishment of Indirect Immunofluorescence Assay for Detection of Foot and Mouth Disease Virus Serotype O[J].Acta Agriculturae Boreali-occidentalis Sinica,2012,21(6):13-16. |
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| Authors: | CAI Kuojun QIAO Jun MENG Qingling CHEN Chuangfu MA Shiwei HUANG Jiong and WEI Jie |
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| Institution: | 1.Department of Animal Science And Technology,Shihezi University,Shihezi Xinjiang 832003,China; 2.Veterinary Research Institute,Xinjiang Academy of Animal Sciences,Urumqi 830000,China) |
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| Abstract: | To establish an indirect immunofluorescence assay(IFA) test to diagnose swine foot-and-mouth disease virus(FMDV) serotype O,the porcine anti-FMDV serotype O serum was served as the primary antibody and the fluorescein isothiocyanated(FITC) labeled Protein A protein(a surface protein of S.aures) as the secondary antibody.This detection method was developed by the optimization of reaction conditions.The optimum conditions of IFA were as follows: the optimum working dilutions of anti-FMDV Serotype O antibody and FITC-labeled protein A were 1∶50 and 1∶800 respectively,the optimum incubating time was 1 hour for both.IFA can specifically detect FMDV serotype O in the BHK-21 cells,while the results were negative in BHK-21 cells infected with FMDV serotype A,AsiaⅠand SVDV.The results showed that the IFA test is specific,sensitive,simple and rapid to detect FMDV serotype O,which may be applied in diagnosis and studies of the localization and dynamic distribution of the FMDV in infected organisms. |
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| Keywords: | Foot-and-mouth disease virus serotype O Indirect immunofluorescence assay |
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