| 小白菜品种的SSR指纹图谱及遗传特异性分析 |
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| 引用本文： | 王笑一,于拴仓,张凤兰,余阳俊,赵岫云,张德双.小白菜品种的SSR指纹图谱及遗传特异性分析[J].华北农学报,2008,23(5). |
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| 作者姓名： | 王笑一 于拴仓 张凤兰 余阳俊 赵岫云 张德双 |
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| 作者单位： | 1. 首都师范大学,生命科学院,北京,100037;北京市农林科学院,蔬菜研究中心,北京,100097
2. 北京市农林科学院,蔬菜研究中心,北京,100097 |
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| 摘 要： | 为研制我国小白菜品种的DUS测试标准,搜集和筛选了形态差异大、不同生态类型的地方品种和目前生产上栽培面积大的杂交一代品种80份,在植物学性状调查的基础上,利用SSR标记对80份小白菜品种进行了遗传特异性分析,构建了SSR指纹图谱.从131对SSR引物中筛选了20对多态引物组合,对80个品种进行了分析,共检测出56个多态性条带,多态性比率73.68%,平均每对引物组合产生2.8个多态性条带.用5对引物Na12E02、Na12A08、Ni4D09、BRMS-269和BRMS-296的组合获得了每个品种的SSR特征带,可以将80个品种区分开来,从而建立80份小白菜品种的SSR指纹图谱.基于SSR标记的聚类分析表明,形态相似或来源相同的品种遗传基础相近,以遗传距离为0.36为划分标准,87.50%的品种具有遗传特异性.
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| 关 键 词： | 小白菜 SSR标记 指纹图谱 |
SSR Fingerprinting and Genetic Distinctness of Pak-choi(Brassica rapa L.ssp.chinensis Makino) |
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| WANG Xiao-yi,YU Shuan-cang,ZHANG Feng-lan,YU Yang-jun,ZHAO Xiu-yun,ZHANG De-shuang.SSR Fingerprinting and Genetic Distinctness of Pak-choi(Brassica rapa L.ssp.chinensis Makino)[J].Acta Agriculturae Boreali-Sinica,2008,23(5). |
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| Authors: | WANG Xiao-yi YU Shuan-cang ZHANG Feng-lan YU Yang-jun ZHAO Xiu-yun ZHANG De-shuang |
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| Institution: | WANG Xiao-yi1,2,YU Shuan-cang2,ZHANG Feng-lan2,YU Yang-jun2,ZHAO Xiu-yun2,ZHANG De-shuang2 (1.College of Life Science,Capital Normal University,Beijing 100037,China,2.Vegetable Reaserch Center,Beijing Academy of Agriculture , Forestry Sciences,Beijing 100097,China) |
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| Abstract: | To develop DUS testing standards for Pak-choi,we collected and selected 80 Pak-choi varieties,which are landraces with different morphology and ecotype and hybrids in a large planting area in China.On the basis of botanic characters in vestigation,SSR fingerprinting and genetic distinctness of 80 non-heading Chinese cabbage varieties were analyzed by a set of SSR markers in this study.Out of SSR primer 131 pairs,20 pairs were screened out with polymorphism among 80 varities and 56 polymorphic bands were obt... |
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| Keywords: | Pak-choi Simple sequence repeats(SSR) Fingerprinting |
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