| 小黄鱼边角料的酶解工艺及酶解液性能研究 |
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| 引用本文： | 丁慧璞,欧阳伟虹,黄玉婷,张捷,王志江,刘利萍.小黄鱼边角料的酶解工艺及酶解液性能研究[J].核农学报,2020,34(9):2021-2031. |
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| 作者姓名： | 丁慧璞 欧阳伟虹 黄玉婷 张捷 王志江 刘利萍 |
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| 作者单位： | 1浙江万里学院生物与环境学院,浙江 宁波 315100; 2浙江医药高等专科学校制药工程学院,浙江 宁波 315100 |
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| 基金项目： | 创新项目;宁波市科技局农业重大项目 |
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| 摘 要： | 为开发利用小黄鱼边角料制备浓缩鱼汤,本研究采用酶解工艺对小黄鱼边角料进行酶解并对其酶解液的功能活性进行研究。以氨基酸态氮含量为指标,通过单因素及响应面试验探究酶制剂种类及添加量、pH值和酶解时间对酶解效率的影响;采用DEAE层析柱对酶解液进行分离纯化,SDS-PAGE凝胶电泳测定酶解多肽的分子量;通过测定酶解多肽对·OH和DPPH自由基的清除率、对细菌生长曲线的影响判断酶解多肽的抗氧化性和抗菌性。结果表明,以碱性蛋白酶为酶制剂,当酶与底物蛋白比为322 U·g-1、固液比(m:v)为1:2、pH值为11.0、温度为55℃、酶解时间为2 h时,小黄鱼酶解液中氨基酸态氮含量为0.545 2 g·100g-1;酶解液经DEAE柱分离得到了Ⅰ、Ⅱ、Ⅲ、Ⅳ四组多肽,其中组分Ⅲ多肽的分子量小于3.3 kDa;当酶解液中氨基酸态氮含量分别为8.62 和25.59 mg·mL-1时,对·OH和DPPH自由基的清除率分别约为80%和61%;组分Ⅲ多肽对枯草芽孢杆菌、酵母菌、金黄色葡萄球菌、大肠杆菌均有不同程度的生长抑制作用。本研究为小黄鱼边角料开发浓缩鱼汤及其他综合利用提供了依据。
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| 关 键 词： | 小黄鱼边角料 酶解工艺 多肽分离纯化 抗氧化 抑菌性 |
| 收稿时间： | 2019-05-07 |
Enzymatic Hydrolysis Technology of Small Yellow Croaker Scraps and Properties of Its Enzymatic Hydrolysis Solution |
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| DING Huipu,OU-YANG Weihong,HUANG Yuting,ZHANG Jie,WANG Zhijiang,LIU Liping.Enzymatic Hydrolysis Technology of Small Yellow Croaker Scraps and Properties of Its Enzymatic Hydrolysis Solution[J].Acta Agriculturae Nucleatae Sinica,2020,34(9):2021-2031. |
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| Authors: | DING Huipu OU-YANG Weihong HUANG Yuting ZHANG Jie WANG Zhijiang LIU Liping |
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| Institution: | 1College of Biological and Environmental Sciences, Zhejiang WanLi University, Ningbo, Zhejiang 315100; 2Department of Pharmaceutical Engineering, Zhejiang Pharmaceutical College, Ningbo, Zhejiang 315100 |
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| Abstract: | In order to develop and utilize the small yellow croaker scraps to prepare concentrated fish soup, the enzymatic hydrolysis of the small yellow croaker scraps was carried out and the functional activity of the hydrolysate was studied. Taking the amino nitrogen content as an index, the effects of enzyme type and dosage, ratio of material to liquid, pH and time on enzyme efficiency were investigated through single factor and response surface tests. DEAE chromatography column was used to separate and purify the enzymatic hydrolysate. SDS-PAGE gel-electrophoresis was used to determine the molecular weight of the enzymatic hydrolysate. The influence of OH and DPPH radical scavenging rate on the growth curve of bacteria was determined. The antioxidant and antibacterial properties were also analyzed. The results showed that the amino nitrogen content in the hydrolysate of small yellow croaker was 0.545 2 g·100 g-1 with alkaline protease as enzyme preparation, the ratio of enzyme to substrate protein was 322 U·g-1, the ratio of solid to liquid (m:v) was 1:2, under the pH value of 11, temperature of 55℃ and time of 2 hours. The enzymatic hydrolysate of small yellow croaker was separated by DEAE column and four groups of polypeptides Ⅰ, Ⅱ, Ⅲ and Ⅳ were obtained, in which the molecular weight of the component Ⅲ polypeptides was less than 3.3 kDa.When the content of amino nitrogen in the hydrolysate was 8.62 and 25.59 mg·mL-1respectively, the scavenging rate of ·OH and DPPH was about 80% and 61%; the component Ⅲ polypeptide had different degrees of growth inhibition on Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Saccharomyce. The study laid theoretical foundation for the development of concentrated fish soup and other comprehensive utilization of small yellow croaker scraps. |
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| Keywords: | small yellow croaker scraps enzymatic hydrolysis process polypeptide separation and purification antioxidant antibacterial activity |
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