| 高温胁迫下硒硫互作调控西兰花芽苗生理及萝卜硫素代谢研究 |
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| 引用本文： | 韩宇,朱振宇,沈敏熙,程雨薇,尹永祺,方维明.高温胁迫下硒硫互作调控西兰花芽苗生理及萝卜硫素代谢研究[J].核农学报,2020,34(6):1350-1358. |
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| 作者姓名： | 韩宇 朱振宇 沈敏熙 程雨薇 尹永祺 方维明 |
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| 作者单位： | 扬州大学食品科学与工程学院,江苏 扬州 225127 |
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| 基金项目： | 江苏省高等学校大学生创新创业训练计划;创新工程项目;青年骨干教师项目;中国博士后科学基金面上项目 |
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| 摘 要： | 为探讨高温胁迫下硒硫互作对西兰花芽苗生理及萝卜硫素代谢的影响,以西兰花籽粒为试验材料,经单独喷施硫酸锌(ZnSO4)、亚硒酸钠(Na2SeO3)、二者联合喷施、以及结合高温胁迫下喷施处理,分析发芽期间西兰花芽苗主要生理生化指标并利用荧光定量PCR技术分析萝卜硫素代谢关键酶基因表达的变化。结果表明,单独施用Na2SeO3可显著增加芽长和芽苗单株鲜重(P<0.05),有效缓解高温及ZnSO4对西兰花芽苗生长发育的抑制作用;发芽期间,相较单独喷施ZnSO4处理,西兰花芽苗经高温后联合喷施ZnSO4及Na2SeO3处理,其硒元素含量、总抗氧化能力、硫代葡萄糖苷含量、黑介子酶活性、异硫氰酸酯含量、萝卜硫素含量均显著提高(P<0.05),其中萝卜硫素含量相较单独喷施ZnSO4处理增加了39%。高温联合ZnSO4及Na2SeO3处理4 d的芽苗中MYB28、UGT74B1及ST5b基因表达量均较对照显著上调,而BoHMT1显著下调(P<0.05)。综上所述,西兰花芽苗经高温联合硒硫处理是富集萝卜硫素的有效方式,该研究结果为生产富含萝卜硫素的功能性芽苗菜提供了理论依据和技术支撑。
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| 关 键 词： | 西兰花 萝卜硫素 高温胁迫 亚硒酸钠 硫酸锌 |
| 收稿时间： | 2019-09-19 |
Effects of Selenium and Sulfur on Physiology and Metabolism of Sulforaphane of Broccoli Sprouts Under High Temperature Stress |
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| HAN Yu,ZHU Zhenyu,SHEN Minxi,CHENG Yuwei,YIN Yongqi,FANG Weiming.Effects of Selenium and Sulfur on Physiology and Metabolism of Sulforaphane of Broccoli Sprouts Under High Temperature Stress[J].Acta Agriculturae Nucleatae Sinica,2020,34(6):1350-1358. |
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| Authors: | HAN Yu ZHU Zhenyu SHEN Minxi CHENG Yuwei YIN Yongqi FANG Weiming |
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| Institution: | College of Food Science and Engineering, Yangzhou University, Yangzhou, Jiangsu 225127 |
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| Abstract: | In order to explore the effects of selenium-sulfur interaction on the physiology and sulforaphane metabolism of broccoli sprouts under high temperature stress, broccoli seeds were sprayed with ZnSO4 or Na2SeO3 alone or combined with ZnSO4 or Na2SeO3 or both under high temperature stress. The main physiological and biochemical indexes of broccoli sprouts during germination were analyzed and the changes of gene expression of key enzymes in sulforaphane metabolism were analyzed by fluorescence quantitative PCR. The results showed that the application of Na2SeO3 alone could significantly increase the sprouts length and the fresh weight per plant (P<0.05), and effectively alleviate the inhibition of high temperature and ZnSO4 on the growth and development of broccoli sprouts. During germination, compared with spraying ZnSO4 alone, the selenium content, total antioxidant capacity, glucosinolates content, mesonase activity, isothiocyanate content and sulforaphane content of broccoli sprouts sprayed with ZnSO4 and Na2SeO3 after high temperature were significantly increased (P<0.05). The content of sulforaphane was 39% higher than that of spraying ZnSO4 alone. The expressions of MYB28, UGT74B1 and ST5b in sprouts treated with high temperature combined with ZnSO4 and Na2SeO3 for 4 days were significantly up-regulated compared with the control, while BoHMT1 was significantly down-regulated (P<0.05). To sum up, broccoli sprouts treated with high temperature combined with selenium and sulfur is an effective way to enrich sulforaphane. The results of this study provide a theoretical basis and technical support for the production of functional sprouts rich in sulforaphane. |
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| Keywords: | broccoli sulforaphane high temperature stress Na2SeO3 ZnSO4 |
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