| 罗汉果皂苷提取物对INS-1胰岛β细胞糖脂毒性的保护作用研究 |
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| 引用本文： | 喻柯柯,刘合生,张境,杨文康,曹少谦,陈秋平,戚向阳.罗汉果皂苷提取物对INS-1胰岛β细胞糖脂毒性的保护作用研究[J].核农学报,2020,34(2):348-355. |
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| 作者姓名： | 喻柯柯 刘合生 张境 杨文康 曹少谦 陈秋平 戚向阳 |
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| 作者单位： | 1浙江万里学院生物与环境学院,浙江 宁波 315100; 2浙江医药高等专科学校食品学院,浙江 宁波 315100 |
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| 基金项目： | 国家自然科学基金;浙江省重中之重学科建设项目 |
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| 摘 要： | 为阐明罗汉果皂苷提取物(MGE)对胰岛β细胞糖脂毒性的保护作用,本试验通过噻唑蓝(MTT)比色法、Hoechst 33342/PI荧光染色法和Western blotting研究MGE对高糖、高脂诱导INS-1胰岛β细胞活力降低、凋亡及促凋亡因子caspase 3表达水平的影响。结果表明,在0.1~100 μg·mL-1浓度范围内,MGE对INS-1细胞无明显细胞毒性(P>0.05)。高糖、高脂处理使INS-1胰岛β细胞活力显著降低(P<0.05),而MGE(0.1~100 μg·mL-1)干预可明显增强INS-1细胞活力,并呈一定的剂量效应关系。与高糖高脂模型组相比,100 μg·mL-1MGE干预使INS-1胰岛β细胞活性上升了15.5%。荧光染色表明,MGE处理明显抑制了高糖、高脂诱导的INS-1胰岛β细胞凋亡。免疫印迹结果表明,高糖、高脂处理的INS-1胰岛β细胞,cleaved caspase 3蛋白表达水平显著上调,而MGE干预显著下调cleaved caspase 3水平(P<0.05)。本研究结果表明,MGE干预改善了胰岛β细胞的糖脂毒性,抑制了细胞凋亡,其抗凋亡作用可能与抑制caspase 3的剪切活化有关。本研究结果为罗汉果高附加值产品的开发及天然、安全降糖功能因子的筛选提供了一定的理论依据。
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| 关 键 词： | 罗汉果皂苷 胰岛β 细胞 糖脂毒性 caspase3 凋亡 |
| 收稿时间： | 2018-08-20 |
Protective Effects of Mogroside Extract on Glucolipotoxicity of INS-1 Pancreatic Islet β Cells |
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| YU Keke,LIU Hesheng,ZHANG Jing,YANG Wenkang,CAO Shaoqian,CHEN Qiuping,QI Xiangyang.Protective Effects of Mogroside Extract on Glucolipotoxicity of INS-1 Pancreatic Islet β Cells[J].Acta Agriculturae Nucleatae Sinica,2020,34(2):348-355. |
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| Authors: | YU Keke LIU Hesheng ZHANG Jing YANG Wenkang CAO Shaoqian CHEN Qiuping QI Xiangyang |
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| Institution: | 1College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang 315100; 2Faculty of Food Science, Zhejiang Pharmaceutical College, Ningbo, Zhejiang 315100 |
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| Abstract: | In order to explore the protective effect of mogroside extract (MGE) on the glucolipotoxicity of pancreatic islet β cells, injury model on INS-1 pancreatic islet β cells with high concentration of glucose (HG) in combination of palmitic acid (PA) were treated with or without MGE. The cell viability, apoptosis and caspase-3 protein level were investigated by the means of 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT),Hoechst 33342/propidium iodide (PI) fluorescent staining and Western blotting assays. The results showed that no apparent cytotoxicity was observed in INS-1 cell with MGE concentration of 0.1 to 100 μg·mL-1. HG in combination with PA caused a large reduction in the cell viability, which can be increased largely by MGE intervention (0.1 to 100 μg·mL-1) in a dose-dependent manner. Furthermore, INS-1 cell viability increased by 15.5% with MGE treatment at a dose of 100 μg·mL-1 compared to injured cells. In addition, results of fluorescent staining indicated MGE treatment notably inhibited the cell apoptosis induced by HG and PA. Accordingly, immunoblotting experiment showed that cleaved caspase 3 level was upregulated obviously, while the MGE treatment significantly downregulated the level of cleaved caspase 3 (P<0.05=. These findings suggest that MGE treatment mitigates glucolipotoxicity of pancreatic islet β cell by the inhibition of cell apoptosis, that the MGE may inhibit the activation of caspase 3. The current study provides a theoretical basis for the developing of value-added products from Siraitia grosvenorii (swingle) and screening bioactive ingredients with safe hypoglycemic activity. |
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| Keywords: | mogroside pancreatic islet β cell glucolipotoxicity caspase 3 apoptosis |
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