| 铁皮石斛PRC2复合体成员的鉴定及对胁迫响应的表达分析 |
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| 引用本文： | 仇汉林,屈东海,陈东红,朱玉球,斯金平.铁皮石斛PRC2复合体成员的鉴定及对胁迫响应的表达分析[J].核农学报,2020,34(3):497-505. |
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| 作者姓名： | 仇汉林 屈东海 陈东红 朱玉球 斯金平 |
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| 作者单位： | 1 浙江农林大学/省部共建亚热带森林培育国家重点实验室/国家林业局铁皮石斛工程技术研究中心,浙江 临安 311300; 2 湖南农业大学生物科学技术学院,湖南 长沙 410128 |
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| 基金项目： | 国家重点研发课题;浙江农林大学科研发展基金 |
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| 摘 要： | 为了探讨PRC2复合体在铁皮石斛生长发育和胁迫响应中的功能,通过生物信息学方法筛选了铁皮石斛PRC2核心成员DcCLF、DcSWN、DcEMF2、DcFIE和DcMSI1,借助酵母双杂交技术分析了它们之间的互作关系,利用半定量PCR分析了PRC2核心成员的组织表达谱,通过荧光定量PCR检测了它们对非生物胁迫(低温、高温、脱水)和病害胁迫(齐整小核菌、灰葡萄孢霉菌)的响应情况。结果表明,铁皮石斛PRC2复合体包含5个成员:E(z)同源基因DcCLF和DcSWN、Su(z)12基因DcEMF2、ESC基因DcFIE、p55基因DcMSI1,且这5个成员间的互作关系基本符合模式植物的互作模式,也存在物种特异性,表明PRC2复合体在进化中既有保守性也有特殊性。PRC2核心成员在铁皮石斛根、茎、叶、花蕾、成花中均有表达,但不同基因的表达存在组织差异性。同时,PRC2成员响应不同的环境和病害胁迫:DcCLF受低温、高温和脱水等各种环境胁迫的显著诱导;DcMSI1和DcEMF2在齐整小核菌侵染下表达明显上调,而DcSWN在灰葡萄孢霉菌侵染下受诱导程度最大。铁皮石斛PRC2复合体在生长发育和胁迫应答中发挥重要作用,可为分子辅助育种提供关键靶标基因。
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| 关 键 词： | 铁皮石斛 PRC2 环境因子 真菌病害 胁迫应答 |
| 收稿时间： | 2019-01-02 |
Identification of PRC2 Subunits of Dendrobium catenatum and Their Responses to Stresses |
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| QIU Hanlin,QU Donghai,CHEN Donghong,ZHU Yuqiu,SI Jinping.Identification of PRC2 Subunits of Dendrobium catenatum and Their Responses to Stresses[J].Acta Agriculturae Nucleatae Sinica,2020,34(3):497-505. |
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| Authors: | QIU Hanlin QU Donghai CHEN Donghong ZHU Yuqiu SI Jinping |
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| Institution: | 1 Zhejiang A & F University/National Forestry Dendrobium Catenatum Engineering Technology Research Center/ State Key Laboratory of Subtropical Silviculture, Lin'an, Zhejiang 311300; 2 College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan 410128 |
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| Abstract: | In order to investigate the functions of PRC2 complex in the development progress and stress response of D. catenatum, the core subunits of D. catenatum PRC2 including DcCLF, DcSWN, DcEMF2, DcFIE and DcMSI1 were screened via bioinformatics method, and the interactions between them were analyzed by yeast two-hybrid assay, the tissue expression profiles of PRC2 core subunits were detected by semi-quantitative PCR, and their responses to abiotic stresses (low temperature, high temperature, and dehydration) and pathogen stresses (Sclerotium rolfsii and Botrytis cinerea) were examined by real-time quantitative PCR. The results showed that the D. catenatum PRC2 complex contains five members: E(z) homologous genes DcCLF and DcSWN, Su(z)12 gene DcEMF2, ESC gene DcFIE, p55 gene DcMSI1. Their interaction relationships were mostly consistent with the mode in the model plant, but species specificity was also present, indicating that the PRC2 complex was both conservative and specific during evolution. The core subunits of PRC2 were widely expressed in the roots, stems, leaves, flower buds, and open flower, but distinct genes displayed discrepancies among different tissues. At the same time, PRC2 subunits were involved in response to different environmental factors and pathogens. DcCLF was significantly induced by various environmental stresses such as low temperature, high temperature and dehydration. DcMSI1 and DcEMF2 were remarkably up-regulated under the infection of Sclerotium rolfsii, while DcSWN was most induced under the infection of Botrytis cinerea. This study suggested that the D. catenatum PRC2 complex plays an important role in developmental program and stress response, which could be used as potential target genes for molecular-assisted breeding. |
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| Keywords: | Dendrobium catenatum PRC2 environmental factors fungal pathogen stress response |
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