| 秋葵微粉对小鼠肠道内环境的影响 |
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| 引用本文： | 辛松林,陈援援,邱秀艳,周慧超,吴玉姣.秋葵微粉对小鼠肠道内环境的影响[J].核农学报,2020,34(4):816-823. |
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| 作者姓名： | 辛松林 陈援援 邱秀艳 周慧超 吴玉姣 |
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| 作者单位： | 四川旅游学院食品学院,四川 成都 610100 |
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| 基金项目： | 四川省大学生创新创业训练计划;川菜发展研究中心项目 |
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| 摘 要： | 为研究不同剂量秋葵微粉对小鼠肠道内环境的影响,将小鼠随机分为4组,即对照组、高剂量组(1 800 mg·kg-1·d-1)、中剂量组(900 mg·kg-1d-1)以及低剂量组(450 mg·kg-1d-1),灌胃6周后,检测其粪便、结肠、盲肠相关的肠道指标。结果表明,与对照组相比,各剂量组小鼠粪便含水量均增加,且高剂量组粪便含水量显著高于对照组(P<0.05);各剂量组粪便pH值均极显著降低(P<0.01);各剂量组盲肠内容物 pH 值和结肠内容物pH值均降低。各剂量组盲肠内容物菌群OTU数量均极显著高于对照组(P<0.01),中剂量组Chaol值显著高于对照组(P<0.05);各剂量组结肠内容物菌群的OTU数量较对照组均有增加,中剂量组和低剂量组Chaol值极显著(P<0.01)和显著(P<0.05)高于对照组。各剂量组盲肠、结肠内容物中短链胎肪酸(SCFA)含量均较对照组增加。秋葵微粉可以提高小鼠肠道中的益生菌比例,降低有害菌比例,促进肠道乙酸、丙酸和丁酸等SCFA含量明显升高。本研究结果为秋葵微粉用于改善动物肠道健康提供了理论依据。
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| 关 键 词： | 秋葵微粉 肠道菌群 短链脂肪酸 结肠 盲肠 粪便 |
| 收稿时间： | 2018-11-02 |
Effect of Okra Micropowder on Intestinal Environment in Mice |
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| XIN Songlin,CHEN Yuanyuan,QIU Xiuyan,ZHOU Huichao,WU Yujiao.Effect of Okra Micropowder on Intestinal Environment in Mice[J].Acta Agriculturae Nucleatae Sinica,2020,34(4):816-823. |
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| Authors: | XIN Songlin CHEN Yuanyuan QIU Xiuyan ZHOU Huichao WU Yujiao |
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| Institution: | College of Food Science, Sichuan Tourism College, Chengdu, Sichuan 610100 |
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| Abstract: | In order to study the effect of okra micropowder on intestinal environment in mice, 40 mice were randomly divided into 4 groups, the control group and okra powder of low(450 mg·kg-1d-1), medium(900 mg·kg-1d-1)and high(1 800 mg·kg-1d-1)dose group. After 6 weeks gavaged administration, the indicators related to the feces, colon and cecum were detected. The results showed that the water content of feces in each dose group increased significantly compared with the control group, and it was significantly higher in high dose group than that in the control group (P<0.05). The pH values of feces each dose group were all decreased significantly (P<0.01); and the pH value of cecal contents and colon contents were also decreased. The number of OTU in the cecal contents microflor of each dose group was significantly higher than that in the control group (P<0.01), the Chaol value of the middle dose group was significantly higher than that of the control group (P<0.05); The number of OTU in the colon contents microflor of each dose group was higher than that in the control group (P>0.05), and the Chaol value in the middle dose group was significantly higher than that in the control group (P<0.01). Short chain falty acid(SCFA) content in cecum and colon contents increased (P>0.05). In addition, okra micropowder could increase the proportion of probiotics in the intestinal tract of mice, reduced the proportion of harmful bacteria, and promoted the content of SCFA, such as acetic acid, propionic acid and butyric acid, and so on. This study provided theoretical basis for okra micropowder could improve the intestinal health in mice. |
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| Keywords: | okra powder intestinal microflora short chain fatty acids colon cecum feces |
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