| 盐胁迫下宁夏枸杞Na+吸收及Na+/H+转运蛋白与H+-ATPase基因表达的研究 |
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| 引用本文： | 梁敏,许兴,丁向真,李志英,郑蕊,杨淑娟,毛桂莲.盐胁迫下宁夏枸杞Na+吸收及Na+/H+转运蛋白与H+-ATPase基因表达的研究[J].核农学报,2020,34(4):745-751. |
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| 作者姓名： | 梁敏 许兴 丁向真 李志英 郑蕊 杨淑娟 毛桂莲 |
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| 作者单位： | 1 宁夏大学生命科学学院,宁夏 银川 750021; 2 宁夏优势特色作物现代分子育种重点实验室,宁夏 银川 750021; 3 宁夏大学西北退化生态系统恢复与重建教育部重点实验室,宁夏 银川 750021 |
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| 基金项目： | 宁夏回族自治区自然科学基金;国家自然科学基金 |
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| 摘 要： | 为从分子水平揭示宁夏枸杞钠的吸收积累机理,本试验采用原子吸收分光光度法和实时荧光定量PCR(RT-qPCR)方法,对盐胁迫下宁夏枸杞根中Na+、K+含量以及质膜和液泡膜Na+/H+转运蛋白与H+-ATPase基因表达水平进行测定分析。结果表明,相同胁迫时间下,随着NaCl处理浓度的增加,枸杞根系中Na+浓度总体呈缓慢增加趋势,K+含量呈先增加后减少趋势,Na+/K+比值呈先减少后增加趋势;编码质膜和液泡膜的Na+/H+转运蛋白基因LbSOS1、LbNHX1以及液泡膜H+-ATPase基因LbVHA-C1表达量均呈升高趋势,质膜H+-ATPase基因LbHA1表达量呈先升高后降低趋势。相同NaCl处理浓度下,随着胁迫时间的延长,Na+含量总体呈增加趋势,K+含量呈先增加后减少趋势,Na+/K+比值呈增加趋势。LbSOS1、LbNHX1表达量总体呈先升高后降低趋势,LbVHA-C1、LbHA1表达量总体呈降低的趋势。相关性分析显示,不同胁迫时间下,枸杞根中LbSOS1、LbNHX1、LbVHA-C1和LbHA1表达量与Na+含量存在一定的正相关或负相关。上述结果表明,在低浓度NaCl胁迫时,维持枸杞体内较高的K+/Na+比值是宁夏枸杞耐盐的主要方式之一,同时也说明在胁迫初期,质膜和液泡膜的Na+/H+转运蛋白与H+-ATPase参与了枸杞细胞中Na+及时排出胞外和区隔于液泡,从而保持了根细胞内Na+的稳定性。此外,随着胁迫时间的延长和NaCl处理浓度的增加,LbSOS1、LbNHX1、LbVHA-C1和LbHA1的表达水平均降低,而 Na+积累量大幅增加,致使枸杞抗盐性降低。本研究揭示了宁夏枸杞的耐盐机理,为利用宁夏枸杞改良宁夏大面积盐碱地提供了理论依据。
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| 关 键 词： | 盐胁迫 实时荧光定量PCR Na+含量 Na+/H+转运蛋白 H+-ATPase |
| 收稿时间： | 2019-01-24 |
Effects of Salt Stress on Na+Uptake and Expression of Na+/H+Transporter and H+-ATPase Genes in Lycium barbarum L. |
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| LIANG Min,XU Xing,DING Xiangzhen,LI Zhiying,ZHENG Rui,YANG Shujuan,MAO Guilian.Effects of Salt Stress on Na+Uptake and Expression of Na+/H+Transporter and H+-ATPase Genes in Lycium barbarum L.[J].Acta Agriculturae Nucleatae Sinica,2020,34(4):745-751. |
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| Authors: | LIANG Min XU Xing DING Xiangzhen LI Zhiying ZHENG Rui YANG Shujuan MAO Guilian |
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| Institution: | 1 School of Life Science, Ningxia University, Yinchuan, Ningxia 750021; 2 Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia, Yinchuan, Ningxia 750021; 3 Key Laboratory for Restoration and Reconstruction of Degraded Ecosystem in North-western China, Yinchuan, Ningxia 750021 |
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| Abstract: | To investigate the mechanism of Na+ uptake and accumulation Lycium barbarum L. The contents of Na+and K+ in the roots of Lycium barbarum L. was determined using atomic absorption spectrophotometry under different salt treatments. Meanwhile, the expression levels of Na+/H+transporter and H+-ATP enzyme involved in ion transport in plasm member and tonoplast were determined by quantitative real time PCR. The results showed that the plant exhibited slow increasing in Na+ content in response to salt stress. However, the K+content increased and then decreased, the ratio of Na+/K+decreased and then increased. The expression abundance of LbSOS1、LbNHX1and LbVHA-C1 in plasm membrane and tonoplast remained relatively higher but LbHA1 increased and then decreased. Long-term stress of NaCl induced the accumulation of Na+ but decreased K+ content, and the ratio of Na+/K+ increased. Meanwhile, The expression abundance of LbSOS1、LbNHX1 increased and then decreased but LbVHA-C1and LbHA1 decreased. Significantly positive or negative correlations was found between the expression level of LbSOS1,LbNHX1,LbVHA-C1and LbHA1 and the content of Na+. That indicated retaining higher ratio of K+/Na+was one of the main ways of salt tolerance in Lycium barbarum L.under low NaCl concentration.The high express level of Na+/H+transporter and H+-ATP enzyme in plasma membrane and vacuolar membrane could excrete Na+ from the cytoplasm into vacuolar cells at the early stage of stress, but long-term time stress induced the lower expression of LbSOS1,LbNHX1,LbVHA-C1and LbHA1 and the accumulation of Na+,which contributed to the reduction of salt tolerance of Lycium barbarum L. The results of this study laid a theoretical foundation for salt-tolerance of Lycium barbarum L. and improvement of saline-alkali soil in large area. |
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| Keywords: | salt stress quantitative real time PCR Na+content Na+/H+transporter H+-ATP enzyme |
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