| 基于nanoUPLC-MS/MS的螺旋藻游离肽组成分析 |
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| 引用本文： | 褚宁宁,陈雨豪,陈娟娟,马斌,陈海敏,骆其君.基于nanoUPLC-MS/MS的螺旋藻游离肽组成分析[J].核农学报,2020,34(4):735-744. |
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| 作者姓名： | 褚宁宁 陈雨豪 陈娟娟 马斌 陈海敏 骆其君 |
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| 作者单位： | 宁波大学,农产品质量安全危害因子与风险防控国家重点实验室,浙江 宁波 315211 |
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| 基金项目： | 国家重点研发项目;现代农业产业技术体系专项资金资助;宁波大学王宽诚幸福基金资助项目;浙江省重大科技专项 |
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| 摘 要： | 为了研究螺旋藻中的多肽成分,以钝顶螺旋藻为原料,采用超滤法提取螺旋藻中的游离肽,利用nanoUPLC-MS/MS和PEAKS Studio分析谱图信息,结合NCBI数据库进行比对和从头测序分析,获得游离肽的结构组成和百分含量信息。结果表明,利用数据库比对法和从头测序法,分别得到可信的游离肽4 485个和20 597个,匹配到的蛋白质有1 036种。数据库比对结果中的游离肽主要为七肽到二十一肽,少量为二十一肽以上;从头测序结果中游离肽主要为二肽到十六肽,少量为十六肽以上。数据库比对结果中百分含量最高的游离肽是十肽,为15.95%;从头测序结果中百分含量最高的是五肽,达到24.09%。本研究结果为螺旋藻蛋白资源的进一步开发和利用提供了依据。
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| 关 键 词： | 螺旋藻 游离肽 数据库搜索 从头测序 nanoUPLC-MS/MS |
| 收稿时间： | 2019-07-05 |
Peptide Profiling of Spirulina platensis Based on nanoUPLC-MS/MS |
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| CHU Ningning,CHEN Yuhao,CHEN Juanjuan,MA Bin,CHEN Haimin,LUO Qijun.Peptide Profiling of Spirulina platensis Based on nanoUPLC-MS/MS[J].Acta Agriculturae Nucleatae Sinica,2020,34(4):735-744. |
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| Authors: | CHU Ningning CHEN Yuhao CHEN Juanjuan MA Bin CHEN Haimin LUO Qijun |
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| Institution: | State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, Zhejiang 315211 |
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| Abstract: | In order to study the peptide components in Spirulina platensis,the free peptides in S. platensis were obtained by extracting under ultrafiltration. The information of peptide was analyzed by nanoUPLC-MS/MS and PEAKS Studio software. NCBI database and de novo sequencing analysis were utilized to analyze the sequences and relative contents of peptides. The results showed that 4,485 and 20,597 credible free peptides were obtained by NCBI database searching and de novo sequencing methods. And 1 036 proteins were matched. The distribution characteristics of free peptides are as follows: the free peptides in the NCBI database search results are mainly heptapeptide to peptide with 21 amino acids, and a few are peptides with more than 21 amino acids; The free peptides obtained with de novo sequencing are mainly dipeptide to hexadeceptide, and a few peptides are beyong the sixteen amino acids.It could be found that decapeptide had the highest percentage with about 15.95% in the database search results. And in the de novo sequencing results, pentapeptide had the highest percentage of 24.09%. In all, the distributions of free peptides in S. platensis could play an important role as basic data in the further development of protein products. |
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| Keywords: | Spirulina platensis free peptide database search de novo sequencing nanoUPLC-MS/MS |
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