| 糙皮侧耳产洛伐他汀发酵培养基的优化 |
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| 引用本文： | 殷朝敏,范秀芝,刘纯友,陈浙娅,马昆,史德芳,程薇,高虹.糙皮侧耳产洛伐他汀发酵培养基的优化[J].核农学报,2020,34(1):139-146. |
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| 作者姓名： | 殷朝敏 范秀芝 刘纯友 陈浙娅 马昆 史德芳 程薇 高虹 |
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| 作者单位： | 1湖北省农业科学院农产品加工与核农技术研究所,湖北 武汉 430064;2国家食用菌加工技术研发分中心,湖北 武汉 430064;3广西科技大学生物与化学工程学院,广西 柳州 545006;4华中农业大学食品科技学院,湖北 武汉 430070 |
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| 基金项目： | 湖北省自然科学基金;湖北省农科院青年基金;国家自然科学基金 |
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| 摘 要： | 为提高糙皮侧耳(Pleurotus ostreatus)液体发酵产洛伐他汀的产量,以高产洛伐他汀的糙皮侧耳菌株P380为研究对象,在单因素试验的基础上采用正交试验优化发酵培养基组成。优化后的培养基为:乳糖24.0 g·L-1,玉米粉2.5 g·L-1,氯化铵2.5 g·L-1,磷酸二氢钾5.0 g·L-1,硫酸镁2.5 g·L-1,初始pH值6.0。通过优化培养基,可显著提高糙皮侧耳发酵培养基中洛伐他汀产量,与原始发酵培养基相比,洛伐他汀产量提高3.15倍,生物量提高1.78倍。本研究结果为糙皮侧耳发酵菌质的合理开发利用奠定了基础。
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| 关 键 词： | 糙皮侧耳 洛伐他汀 发酵优化 正交试验 |
| 收稿时间： | 2018-11-06 |
Optimization of the Fermentation Medium for Lovastatin Production in Pleurotus ostreatus |
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| YIN Chaomin,FAN Xiuzhi,LIU Chunyou,CHEN Zheya,MA Kun,SHI Defang,CHENG Wei,GAO Hong.Optimization of the Fermentation Medium for Lovastatin Production in Pleurotus ostreatus[J].Acta Agriculturae Nucleatae Sinica,2020,34(1):139-146. |
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| Authors: | YIN Chaomin FAN Xiuzhi LIU Chunyou CHEN Zheya MA Kun SHI Defang CHENG Wei GAO Hong |
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| Institution: | 1Institute of Agro-Products Processing and Nuclear-Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan, Hubei 430064;2National Research and Development Center for Edible Fungi Processing, Wuhan, Hubei 430064;3College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, Guangxi 545006;4Collegy of Food Science and Technology, Huazhong Agriculture University, Wuhan, Hubei 430070 |
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| Abstract: | In order to improve the yield of lovastatin production in Pleurotus ostreatus strain P380 by liquid fermentation, the composition of the fermentation medium was optimized using orthogonal experiment design based on single factor experiments. The optimized fermentation medium composition were as follows (g·L-1): lactose 24.0, cornmeal 2.5, ammonium chloride 2.5, potassium dihydrogen phosphate 5.0, magnesium sulfate 2.5, and initial pH 6.0. After optimization of the fermentation medium composition, the lovastatin content and biomass increased by 3.15 times and 1.78 times respectively compared to the original fermentation medium. This study provides some technique support for the comprehensive utilization and the development of functional nutritious from P. ostreatus. |
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| Keywords: | Pleurotus ostreatu lovastatin fermentation optimization orthogonal experiment |
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