| 鳖甲胶原蛋白酶解物的分离纯化及体外抗氧化性研究 |
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| 引用本文： | 卢梦楠,李兰芳,景玉蕾,宋伟,李彩燕,钱国英.鳖甲胶原蛋白酶解物的分离纯化及体外抗氧化性研究[J].核农学报,2020,34(5):1047-1053. |
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| 作者姓名： | 卢梦楠 李兰芳 景玉蕾 宋伟 李彩燕 钱国英 |
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| 作者单位： | 浙江万里学院生物与环境学院,浙江 宁波 315000 |
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| 基金项目： | 浙江省自然科学基金;国家自然科学基金;浙江省基础公益研究计划项目;创新项目 |
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| 摘 要： | 为建立中华鳖背甲中胶原蛋白酶解物(CH)的制备条件,并明确其体外抗氧化活性,本研究采用单因素试验探讨鳖甲胶原蛋白酶解物的分离条件。结果表明,其最佳提取工艺参数:胃蛋白酶添加量 4 000 U·g-1、pH值2.5、酶解温度35℃。通过透析纯化后获得体外抗氧化活性最好的组分为分子量<50 kDa的样品。该样品经Sephadex G-75凝胶色谱分离纯化得到2个组分,其中GP-2组分对O2-·、OH·和DPPH·3种自由基清除能力最强;之后对GP-2组分进行离子交换层析分离同样获得2个组分,其中P1组分体外抗氧化活性高于GP-2,其对DPPH·、OH·、O2-·清除率分别为81.67%、65.45%和6.78%。经SDS-PAGE电泳确定P1组分的分子量约40 kDa。本研究结果为鳖甲的开发利用及鳖源新型抗氧化物质的研发提供了一定的理论基础。
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| 关 键 词： | 鳖甲 胶原蛋白酶解物 分离纯化 层析 抗氧化 |
| 收稿时间： | 2018-12-13 |
Isolation,Purification and Antioxidant of Collagen Enzymatic Hydrolysate From the Carapace of Chinese Soft-shelled Turtle (Pelodiscus sinensis) |
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| LU Mengnan,LI Lanfang,JING Yulei,SONG Wei,LI Caiyan,QIAN Guoying.Isolation,Purification and Antioxidant of Collagen Enzymatic Hydrolysate From the Carapace of Chinese Soft-shelled Turtle (Pelodiscus sinensis)[J].Acta Agriculturae Nucleatae Sinica,2020,34(5):1047-1053. |
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| Authors: | LU Mengnan LI Lanfang JING Yulei SONG Wei LI Caiyan QIAN Guoying |
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| Institution: | College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang 315100 |
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| Abstract: | The objective of the present study was to optimize the extraction condition of collagen hydrolysates (CH) from the carapace of Chinese soft-shelled turtle (Pelodiscus sinensis) and to evaluate its antioxidant activities in vitro. The results showed that by using single factor experiment the optimum condition was established when pepsin addition, pH value and enzymatic hydrolysis temperature were 4 000 U?g-1, 2.5, and 35℃, respectively. After dialysis, the CH fraction with molecular weight of less than 50 kDa was obtained and was shown to have the best antioxidant activity. Then this fraction was separated into 2 fractions by Sephadex G-75 gel chromatography, among which GP-2 fraction has the highest free radical (DPPH·, OH· and O2-·) scavenging ability, Afterwards, another 2 fractions were also obtained from GP-2 fraction by ion exchange chromatography. The in vitro antioxidant activity of P1 fraction was higher than that of GP-2 and the free radical (DPPH·, OH· and O2-· scavenging rates of P1 were 81.67%, 65.45% and 6.78%, respectively. The molecular weight of collagen hydrolysate P1 fraction was determined to be 40 kDa by SDS-PAGE. This study provided the theoretical basis for the development and utilization of turtle carapace as well as the researches for new antioxidants from turtles. |
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| Keywords: | turtle carapace collagen enzymatic hydrolysate isolation and purification gel chromatography antioxidant |
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