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| 用农杆菌将Bt基因导入水稻愈伤组织的研究 | |
| 引用本文： | 贝丽霞,王守德,史芝文,徐仲,郭三堆.用农杆菌将Bt基因导入水稻愈伤组织的研究[J].吉林农业大学学报,2000,22(4):36-40. |
| 作者姓名： | 贝丽霞 王守德 史芝文 徐仲 郭三堆 |
| 作者单位： | 1. 黑龙江八一农垦大学植物科技学院黑龙江密山 158308 2. 东北农业大学生物工程系哈尔滨 150030 3. 中国农业科学院生物技术研究中心北京 100081 |
| 摘 要： | 从水稻成熟胚诱导出的愈伤组织经继代培养后可以产生大量的胚性愈伤组织。通过采用农杆菌共培养法转化上述胚性愈伤组织，在附加羧苄青霉素Ｃｂ（５００ｕｇ／ｍＬ）和卡那霉素Ｋｍ（５０ｕｇ／ｍＬ）的选择培养基上进行抗性愈伤组织的筛选，在附加羧苄青霉素Ｃｂ（５０ｕｇ／ｍＬ）和卡那霉素Ｋｍ（２５ｕｇ／ｍＬ）的分化培养基上诱导抗性芽。ＧＵＳ酶活性检测和ＰＣＲ检测结果均为阳性，证明外源目的基因（Ｂｔ）已在寒地水稻抗性愈伤组织中整合并表达。抗性再生植株的ＧＵＳ酶活性及ＰＣＲ检测正在进行中。 |
| 关 键 词： | 水稻 农杆菌 共培养 Bt基因 遗传转化 |
| 文章编号： | 1000-5684(2000)04-0036-05 |
| 修稿时间： | 2000年3月13日 |
Agrobacterium tumefaciens Bt Gene Transfer in Embryogenic Calli of Rice |
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| BEI Li-xia,WANG Shou-de,SHI Zhi-wen,XU Zhong,GUO San-Dui.Agrobacterium tumefaciens Bt Gene Transfer in Embryogenic Calli of Rice[J].Journal of Jilin Agricultural University,2000,22(4):36-40. | |
| Authors: | BEI Li-xia WANG Shou-de SHI Zhi-wen XU Zhong GUO San-Dui |
| Abstract: | Many embryogenic calli were produced by subculture of calli induced from mature embryo of rice. The embryogenic calli were transformed after incubating the calli with Agrobacterium tumefaciens, and the resistant calli were selected on selectivemedia containing Cb (500 μg/ mL)and Km (50 μg/mL). The resistant shoots were induced on differentiation media containing Cb (500μg/mL) and Km (25 μg/mL). Finally, the regenerative plantlets were gained. Gus enzyme activity test and PCR test showed that the foreign gene(Bt)had been integrated and expressed in Oryza sativa L. GUS enzyme activity test and PCR test of resistant plantlets is in progress. |
| Keywords: | Oryza sativa L Agrobacterium tuumefaciens co-cultivation bacillus thuringiensis gene genetic transformation |
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