| 北京鸭群体遗传多样性及遗传资源分析 |
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| 引用本文： | 杨宇泽,侯卓成,常卓,张权.北京鸭群体遗传多样性及遗传资源分析[J].中国畜牧杂志,2019(5):35-40. |
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| 作者姓名： | 杨宇泽 侯卓成 常卓 张权 |
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| 作者单位： | 1.北京市畜牧总站;2.中国农业大学动物科学技术学院;3.广东海洋大学农学院 |
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| 基金项目： | 国家水禽产业体系(CARS-42);2018年度畜禽遗传资源保护与利用项目(PXM2018_036213_000003);广东海洋大学博士启动项目(101402/R1703) |
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| 摘 要： | 为研究北京鸭群体的遗传结构及遗传多样性,实验分别采集北京鸭F_0(120只)、F_1(100只)和F_2世代(100只)的血液样本,采用荧光标记毛细管电泳方法对北京鸭11个微卫星座位进行分析。结果表明:11个微卫星座位在北京鸭F_0、F_1和F_2世代的平均等位基因/有效等位基因数量分别为4.6/2.5、3.5/1.9和4.5/2.2个;平均多态信息含量分别为0.441 3、0.303 8和0.415 0;平均期望杂合度分别为0.487 7、0.363 3和0.454 2;北京鸭3个世代间的遗传变异指标差异不显著。近交系数及基因流分析结果表明,北京鸭群体3个世代近交系数较低,群体内近交系数为0.086 9,微卫星位点的平均基因流为0.934 1。总体来看,北京鸭群体遗传分化程度低,保持了北京鸭群体遗传多样性。
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| 关 键 词： | 北京鸭 微卫星 遗传多样性 遗传资源 |
Study of Beijing Duck Population Genetic Diversity and Resources |
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| YANG Yu-ze,HOU Zhuo-cheng,CHANG Zhuo,ZHANG Quan.Study of Beijing Duck Population Genetic Diversity and Resources[J].Chinese Journal of Animal Science,2019(5):35-40. |
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| Authors: | YANG Yu-ze HOU Zhuo-cheng CHANG Zhuo ZHANG Quan |
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| Institution: | ,Beijing General Station of Animal Husbandry,College of Animal Science and Technology, China Agricultural University,Agricultural College, Guangdong Ocean University |
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| Abstract: | To study the genetic structure and diversity of Beijing duck, we collected 120, 100 and 100 blood samples from F_0, F_1 and F_2 generations of Beijing duck. The polymorphism of 11 microsatellite loci in Beijing duck was analyzed by fluorescence labeled capillary electrophoresis. Results showed that the average of alleles/effective alleles in F_0, F_1 and F_2 generation of 11 microsatellite loci were 4.6/2.5, 3.5/1.9 and 4.5/2.2, respectively, the average of polymorphic information content(PIC) was 0.441 3, 0.303 8 and 0.415 0 and the average of expected heterozygosity(He) was 0.4877, 0.3633 and0.4542, respectively. There were no significant difference in genetic variation indexes among three generations(P>0.05). The results of genetic relationships showed that the inbreeding coefficient of three generations of Beijing duck was 0.0869 and the average of gene flow was 0.9341. These results indicated that the genetic differentiation among the Beijing duck population was low, which maintains the genetic diversity of Beijing duck. |
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| Keywords: | Beijing duck Microsatellite loci Genetic diversity Genetics resources |
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