| 绵羊KISS1基因组织表达及其多态性与产羔数关联分析 |
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| 作者单位： | ;1.中国农业科学院北京畜牧兽医研究所农业部动物遗传育种与繁殖重点实验室;2.甘肃农业大学动物科学技术学院;3.临沂大学农林科学学院 |
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| 摘 要： | 为研究KISS1基因在常年发情的小尾寒羊和季节性发情的草地型藏羊中的表达模式,以及KISS1基因多态性与绵羊繁殖之间的关系,实验采用qPCR技术对比分析KISS1基因在2个品种绵羊的10种繁殖相关组织中的表达差异,同时利用Sequenom MassARRAY~?SNP技术对常年发情绵羊(小尾寒羊、策勒黑羊和湖羊)和季节性发情绵羊(滩羊、苏尼特羊和草地型藏羊)KISS1基因2个SNPs位点多态性进行检测,并与小尾寒羊产羔数进行关联分析。qPCR结果显示:KISS1基因在小尾寒羊下丘脑、大脑、垂体和甲状腺中的表达量高于草原型藏羊(P<0.05);分型结果表明,g.1317523C>T位点基因型频率和等位基因频率在常年发情和季节性发情绵羊品种间差异均达到极显著水平(P<0.01);群体遗传学分析表明,g.1317523C>T位点在6个绵羊群体中均表现为中度多态(0.25T位点在小尾寒羊、滩羊、苏尼特羊和湖羊中均处于哈代温伯格平衡状态(P>0.05),g.1311578G>T位点在苏尼特羊和湖羊中处于哈代温伯格平衡状态(P>0.05);关联分析表明,这2个SNPs位点与小尾寒羊前3胎产羔数均无显著关联(P>0.05),但g.1311578G>T位点TT型各胎产羔数均大于GT和GG型。综上,KISS1基因与绵羊的季节性繁殖密切相关,并且g.1311578G>T位点对绵羊产羔性状有潜在调控作用。
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| 关 键 词： | 绵羊 KISS1基因 组织表达 SNP分型 季节性繁殖 产羔数 |
Tissue Expression and Polymorphism of Sheep KISS1 Gene and Their Association with Litter Size |
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| Institution: | ,Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences,College of Animal Science and Technology, Gansu Agricultural University,College of Agriculture and Forestry Science, Linyi University |
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| Abstract: | In order to study the expression pattern of KISS1 gene in year-round estrous Small Tail Han sheep and seasonal estrous Prairie Tibetan sheep, and explore the relationship between the polymorphism and breeding in sheep. QPCR was performed to investigate the expression of KISS1 gene in 10 reproductive tissues at different breeds of sheep. Year-round estrous(Small Tail Han sheep, Cele black sheep and Hu sheep) and seasonal estrous(Tan sheep, Sunite sheep and Prairie Tibetan sheep) sheep breeds were selected and Sequenom MassARRAY~?SNP assay was applied to genotype two loci of KISS1 gene. Then the association was analyzed between KISS1 and litter size in Small Tail Han sheep. The results of gene expression showed that the KISS1 gene was higher in hypothalamus, brain, pituitary and thyroid of Small Tailed Han sheep than that in Prairie Tibetan sheep(P<0.05). From the result of genotyping, both genotype frequency and allele frequency were extremely different between year-round estrous and seasonal estrous sheep(P<0.01) at g.1317523C>T locus. Population genetic analysis showed that the g.1317523C>T locus had moderate polymorphism(0.25T locus of Small Tail Han sheep, Tan sheep, Sunite sheep and Hu Sheep was under Hardy-Weinberg equilibrium(P>0.05), and the g.1311578G>T locus of Sunite sheep and Hu sheep was under HardyWeinberg equilibrium(P>0.05). Association analysis indicated that the polymorphism of two SNPs had no correlation with the litter size in Small Tail Han sheep(P>0.05), while the litter size of TT was higher than GT and GG in the first three births at g.1317523C>T locus. Therefore, KISS1 gene is closely related to the seasonal reproduction of sheep, and the g.1311578G>T locus might provide a basis for litter size trait selection in sheep. |
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| Keywords: | Sheep KISS1 gene Tissue expression SNP genotyping Seasonal reproduction Litter size |
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