| 采收时间对蜡蚧轮枝菌分生孢子的萌发及致病力的影响 |
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| 引用本文： | 于蓉蓉,石旺鹏,闫跃英,张龙.采收时间对蜡蚧轮枝菌分生孢子的萌发及致病力的影响[J].植物保护,2004,30(5):41-43. |
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| 作者姓名： | 于蓉蓉 石旺鹏 闫跃英 张龙 |
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| 作者单位： | 中国农业大学农业部生物防治重点开放实验室,北京,100094 |
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| 摘 要： | 将蜡蚧轮枝菌Vp菌株在PDA培养基上23℃黑暗培养,分别于第5、15、20、25、30天后采收。将不同时间采收的分生孢子置于2％葡萄糖培养液内培养,16 h后检测其萌发率,结果表明,采收于第5天和第15天的分生孢子萌发率分别达到89.76％和94.98％,显著高于采收于第25天(30.27％)和第30天(6.12％)的孢子的萌发率;在PDA培养基培养5 d和15 d的分生孢子在相同浓度下对温室白粉虱的致病力分别达到83.40％和74.44％,显著高于培养了30 d的分生孢子的致病力(8.76％)。
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| 关 键 词： | 有害生物生物防治 蜡蚧轮枝菌 萌发率 致病率 白粉虱 |
Influence of culture age on germination and virulence of Verticillium lecanii to Trialeurodes vaporiorum |
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| YU Rong-rong;SHI Wang-peng;YAN Yue-ying;ZHANG Long.Influence of culture age on germination and virulence of Verticillium lecanii to Trialeurodes vaporiorum[J].Plant Protection,2004,30(5):41-43. |
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| Authors: | YU Rong-rong;SHI Wang-peng;YAN Yue-ying;ZHANG Long |
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| Institution: | Key Laboratory of Bio-Control;China Agricultural University 100094;China |
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| Abstract: | Verticiiiium lecanii(Zim.)Viegas used in these experiments was strain Beijing-P, isolated from Trialeurodes vapariorum.Spores were cultured at 23℃ on PDA in total darkness and were harvested at 5 d, 15 d, 20 d, 25 d or 30 d with 2% glucose solution. The germination rate of spores and the length germ tube were examined under microscope. The results showed that the germination rate of spores harvested after 5 d and 15 d were 89.76% and 94.98% respectively, which was obviously higher than that harvested after 25 d (30.27%) and 30d (6.12%). The results of bioassay showed infection of the sopres harvested after 5 d and 15 d, reached 83.40% and 74.44% respectively, significantly different from that harvested after 30 d (8.76%). Therefore, culture age is key factor to germination and infection of spores. |
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| Keywords: | biocontrol of pest VerticiLlium Lecanii germination rate virulence Trialeurodes vaporiorum |
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