| 玉树组织培养中的器官形成与植株再生(英文) |
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| 引用本文： | 刘永立,龙自立,高月,原田隆.玉树组织培养中的器官形成与植株再生(英文)[J].浙江大学学报(农业与生命科学版),2007,33(6):591-596. |
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| 作者姓名： | 刘永立 龙自立 高月 原田隆 |
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| 作者单位： | 1. 浙江大学,农业与生物技术学院,浙江,杭州,310029
2. 北海道大学,农学部,日本,札幌,060 |
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| 摘 要： | 以玉树的茎、叶、根为外植体,研究不同培养基和植物生长调节剂对其再生的影响.结果表明,在附加0.1～10 μmol·L-1 NAA和10 μmol·L-1 BA的MS培养基上,不定芽形成率达到100%,每1个外植体再生芽数达到10个以上.将再生芽转接到含有1 μmol·L-1 NAA或者1 μmol·L-1 IBA的1/2MS培养基上,发育成健壮小植株.生根植株移栽到大田,生长良好,8个月后株高达27 cm.
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| 关 键 词： | 玉树 植物生长调节剂 再生芽 |
| 文章编号： | 1008-9209（2007）06-0591-06 |
| 收稿时间： | 2006-12-20 |
| 修稿时间： | 2006年12月20 |
Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens |
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| LIU Yong-li,LONG Zi-li,GAO Yue,Takashi Harada.Organ formation and plant regeneration in vitro tissue culture of Crassula arborescens[J].Journal of Zhejiang University(Agriculture & Life Sciences),2007,33(6):591-596. |
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| Authors: | LIU Yong-li LONG Zi-li GAO Yue Takashi Harada |
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| Abstract: | Effects of medium and plant growth regulators on shoot regeneration from stem, leaf and root explants of Crassula arborescens were studied. Adventitious buds developed into shoots, shoot regeneration rate reached 100% and shoot number was above 10 per explant on the Murashige and Skoog basal medium (MS) supplemented with 0. 1-10 μmol. L-1 α-naphthaleneacetic acid (NAA) and 10 μmol. L-1 N-6-benzyladenine (BA). Rooted shoots grew into vigorous plantlets after transferred to 1/2 MS medium containing 1 μmol. L-1 NAA or 1 μmol. L-1 indolebutyric acid (IBA). The rooted plantlets were transplanted to the field, grew very vigorous and reached 27 cm height after 8 months. |
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| Keywords: | Crassula arborescens plant growth regulators shoot |
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