| 狗牙根平脐蠕孢菌生物学特性及药剂筛选 |
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| 引用本文： | 王建光,孔宝华,王琳,陈海如,欧阳建霞.狗牙根平脐蠕孢菌生物学特性及药剂筛选[J].云南农业大学学报,2007,22(2):202-207. |
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| 作者姓名： | 王建光 孔宝华 王琳 陈海如 欧阳建霞 |
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| 作者单位： | 农业生物多样性与病害控制教育部重点实验室云南农业大学省植物病理重点实验室 云南昆明650201(王建光，孔宝华，陈海如，欧阳建霞)，云南林业职业技术学院 云南昆明650224(王琳) |
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| 基金项目： | 云南省教育厅基金资助(A2002007)，云南省科技攻关(2003NG01)，昆明市科技局(200201009) |
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| 摘 要： | 对狗牙根平脐蠕孢菌的生物学特性及5种杀菌剂对该病原菌的抑制作用进行了研究。结果表明：狗牙根平脐蠕孢菌对多种碳、氮源都能利用,以蔗糖和硝酸钾、L-天冬酰胺最好,但以脲为氮源的培养基生长较差。菌丝最佳生长温度是25~30 ℃之间，在25 ℃时最利于产孢,菌丝生长和分生孢子的致死温度是56 ℃；该菌在pH 3~11范围内均能生长，产生分生孢子，菌丝生长的最适pH 5~7，产孢量最大的pH是 3，9，10；糖浓度在2%~8%菌丝能生长，在糖浓度为2%时菌丝生长最好，产孢量最高；光照对病原菌的菌丝生长和产孢有明显影响,在全光照的条件下有利于菌丝的生长，但不会产孢，黑暗12h光照12h既利于菌丝生长又利于产孢，而全黑暗的条件下不利于菌丝生长和产孢。对5种杀菌剂的研究表明：退菌特和百菌清对狗牙根平脐蠕孢菌菌丝生长的抑制效果较好，其它杀菌剂也能有效的抑制菌丝的生长。
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| 关 键 词： | 海里康 狗牙根平脐蠕孢 生物学特性 药剂筛选 |
| 文章编号： | 1004-390X（2007）02-0202-06 |
| 收稿时间： | 2006-6-12 |
| 修稿时间： | 2006-06-12 |
Biological Characteristics of Bipolaris cynodontis and Fungicide Screening |
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| WANG Jian-guang,KONG Bao-hua,WANG Lin,CHEN Hai-ru,OUYANG Jian-xia.Biological Characteristics of Bipolaris cynodontis and Fungicide Screening[J].Journal of Yunnan Agricultural University,2007,22(2):202-207. |
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| Authors: | WANG Jian-guang KONG Bao-hua WANG Lin CHEN Hai-ru OUYANG Jian-xia |
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| Institution: | 1. Ministry of Education Key Laboratory of Agriculture Biodiverstiy for Plant Disease Management, Key Laboratory of Plant Pathology, Y A U, Kunming 650201, China; 2. Yunan Foresty Vocational Technology Academy, Kunming 650224, China |
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| Abstract: | Biological characterization and the effect of 5 kinds of fungicide of Bipolaris cynodontis were studied. The results showed that the pathogen grew well on the media using sucrose and KNO3 and L-Asparagine, but did not grow well on urea as nitrogen sources. The most suitable range of temperature is 25~30 ℃ and lethal temperature is 56 ℃ for hypha growth, the most suitable temperature is 25 ℃ for produce more conidiospore and lethal temperature is 56 ℃ for conidiospore. Within the pH range of 3~11 the hypha could grow and produce conidiospore. The optimum pH is 5~7 for hypha growth and 3,9,10 for produce more conidiospore. The range of glucose concentration is 2%~8% for hypha growth, the most suitable glucose concentration is 2% for hypha growth and produce more conidiospore. The effect of illumination on Bipolaris cynodontis was significance to the growth hypha and spore numbers. The whole light is advantage of hypha growth, but could not produce conidiospore. Alternation of light and darkness for 12 hours is advantage of hypha growth, as well as produce conidiospore. The whole darkness is not advantage of hypha growth and produce conidiospore. The inhibiting test of 5 kinds of fungicide showed Tuijunte and Chlorothalonil are most effective, and the inhibting effect of the others was well to hypha growth. |
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| Keywords: | Heliconia Bipolaris cynodontis biological characterization screening of fungicides |
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