| 赤眼鳟Toll样受体9基因的cDNA全长克隆及表达特性分析 |
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| 引用本文： | 金生振,王荣华,周智愚,刘巧林,彭慧珍,肖调义.赤眼鳟Toll样受体9基因的cDNA全长克隆及表达特性分析[J].湖南农业大学学报(自然科学版),2017,43(3). |
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| 作者姓名： | 金生振 王荣华 周智愚 刘巧林 彭慧珍 肖调义 |
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| 作者单位： | 1. 湖南农业大学湖南省特色水产资源利用工程技术研究中心,湖南 长沙,410128;2. 湖南农业大学湖南省特色水产资源利用工程技术研究中心,湖南 长沙 410128;水产高效健康生产湖南省协同创新中心,湖南 常德 415000 |
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| 基金项目： | 国家自然科学基金面上项目 |
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| 摘 要： | 为探究赤眼鳟(Squaliobarbus curriculus)Toll样受体9基因(toll–like receptor 9,ScTLR9)的结构特性及其参与免疫应答草鱼呼肠弧病毒(grass carp reovirus,GCRV)感染时的表达特性,采用RACE技术克隆Sc TLR9基因c DNA全长序列,采用实时荧光定量PCR技术比较分析ScTLR9免疫应答GCRV的表达特性。结果显示:ScTLR9的cDNA全长为3 687 bp,编码1 059个氨基酸,有17个富含亮氨酸重复序列(leucine rich repeats,LRRs)结构域。ScTLR9在被检测的9个组织中均有表达,在中肾和头肾中的表达量最高;感染GCRV后,基因TLR9在赤眼鳟和草鱼头肾中的表达量均上调,在赤眼鳟头肾中的表达量于感染后12 h达到峰值,推测TLR9参与了赤眼鳟抗GCRV的免疫应答,且在抗GCRV入侵免疫反应中赤眼鳟较草鱼的免疫应答更为迅速。
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| 关 键 词： | 赤眼鳟 Toll样受体9基因 草鱼呼肠弧病毒 草鱼 |
Molecular cloning and expression characteristics of toll-like receptor gene 9 inSqualiobarbus curriculus |
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| Abstract: | In order to investigate the structural characteristics of the toll-like receptor gene 9 in Squaliobarbus curriculus (ScTLR9), as well as its expression feature on the immune response in case of GCRV infection, the full-length cDNA of gene ScTLR9 was cloned using RACE technology in the study, and the expression ofScTLR9 onimmune response in GCRV infection was analyzed using real-time qPCR. The results showed that the full-length cDNA of ScTLR9 was 3687 bp encoding with a polypeptide of 1059 amino acid residues and containing 17 LRR domains. The ScTLR9 mRNA was expressed in all collected tissues, and the relative expression level in mesonephros and head kidney were higher than that in other tissues; Under GCRV infection, the expression of TLR9 were up-regulated in head kidney for barbel chub and grass carp, the relative expression level reached its peak in 12 h for ScTLR9 in head kidney of barbel chub. It was speculated that the gene ScTLR9 was more quickly on the immune response against GCRV invasion in toll-like receptor than that in grass carp, it played an important role on the response to GCRV infection. |
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| Keywords: | Squaliobarbus curriculus toll-like receptor 9(TLR9) grass carp reovirus (GCRV) Ctenopharyngodon idella |
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