| 芍药查尔酮异构酶基因CHI的表达特性分析 |
| |
| 引用本文： | 吴彦庆,姜宇,赵大球,陶俊.芍药查尔酮异构酶基因CHI的表达特性分析[J].湖南农业大学学报(自然科学版),2017,43(4). |
| |
| 作者姓名： | 吴彦庆 姜宇 赵大球 陶俊 |
| |
| 作者单位： | 1. 扬州大学动物科学与技术学院,江苏扬州,225009;2. 扬州大学园艺与植物保护学院,江苏扬州,225009;3. 扬州大学动物科学与技术学院,江苏扬州 225009;扬州大学园艺与植物保护学院,江苏扬州 225009 |
| |
| 基金项目： | 国家自然科学基金，江苏省高校自然科学研究重大项目 |
| |
| 摘 要： | 为了揭示查尔酮异构酶基因(chalcone isomerase gene,CHI)在芍药花瓣中的表达规律与特点,以不同芍药托桂型品种(‘金辉’‘彤云金焰’‘红楼锦菊’)4个不同发育时期(花蕾期、初开期、盛开期、衰败期)中的内、外花瓣为对象,用qPCR分别检测CHI基因的表达水平,分析其在不同芍药品种、不同发育时期以及内外花瓣之间的表达差异。结果显示:不同发育时期同一品种芍药花瓣组织CHI基因的表达量存在一定差异,从花蕾期到衰败期整体表现为上升趋势;不同品种同一发育时期‘彤云金焰’花瓣组织(内瓣和外瓣)CHI基因的表达水平明显高于‘金辉’和‘红楼锦菊’的;同一芍药品种在相同发育时期内瓣组织的CHI基因表达量均明显高于外瓣组织的。鉴于不同芍药品种、不同发育时期内外花瓣间颜色存在差异,推测CHI基因表达量可能与芍药花瓣颜色的形成有关。该结果可为深入研究芍药CHI功能并开展芍药花色遗传改良分子育种研究提供技术支撑。
|
| 关 键 词： | 芍药 查尔酮异构酶基因CHI 花瓣 基因表达 |
Expression pattern analysis on the chalcone isomerase (CHI) gene inPaeonia lactiflora cultivars |
| |
| WU Yanqing,JIANG Yu,ZHAO Daqiu,TAO Jun.Expression pattern analysis on the chalcone isomerase (CHI) gene inPaeonia lactiflora cultivars[J].Journal of Hunan Agricultural University,2017,43(4). |
| |
| Authors: | WU Yanqing JIANG Yu ZHAO Daqiu TAO Jun |
| |
| Abstract: | Chalcone isomerase (CHI) is a key gene in regulating the formation of yellow petals. Inner and outer petals from differentPaeonia lactiflora cultivars including Jinhui, Tongyunjinyan, Hongloujinjuat four growing stages (flower-bud stage, initiating bloom stage, bloom stage, wither stage) were selected as materials to study expression pattern and characteristic ofCHI gene. Expression difference ofCHIgene in inner and outer petal at four growing stages among differentPaeonia lactiflora cultivars were conducted using qPCR technology. The results showed that the expression level ofCHI gene exhibited difference in petal tissues from the same cultivar and showed a rise trend from flower bud stage to wither stage; For different cultivars, the expression level ofCHI gene in petal tissues of Tongyunjinyan was apparently higher than that of Jinhuiand Tongyunjinyan at the same growing stages, furthermore, it was apparently higher in inner petals than that in outer petals for the same cultivars at same growing stages. Considering color difference in inner and outer petals at different growing stages and differentPaeonia lactiflora cultivars, it was speculated that the expression level of CHIgene might be related to the color formation of petals inPaeonia lactiflora. The result could provide theoretical guidance for further research on theCHI function and genetic improvement to flower color of Paeonia lactiflora. |
| |
| Keywords: | Paeonia lactiflora chalcone isomerase (CHI) gene petal gene expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《湖南农业大学学报(自然科学版)》浏览原始摘要信息 |
| 点击此处可从《湖南农业大学学报(自然科学版)》下载免费的PDF全文 |
|
