| 桑白皮总黄酮的提取工艺优化及不同方法获取桑白皮的总黄酮提取效果 |
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| 引用本文： | 罗国庆,吴亦集,王振江,卢志菁,唐翠明,沈光林.桑白皮总黄酮的提取工艺优化及不同方法获取桑白皮的总黄酮提取效果[J].蚕业科学,2010,36(5):738-742. |
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| 作者姓名： | 罗国庆 吴亦集 王振江 卢志菁 唐翠明 沈光林 |
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| 作者单位： | 广东省农业科学院蚕业与农产品加工研究所,广州,510610;广东中烟工业公司,广州,510145 |
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| 基金项目： | 广东省科技基础条件建设项目，广东中烟工业公司科技项目，国家科技支撑计划项目 |
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| 摘 要： | 桑白皮中含有的黄酮类化合物具有多种药用活性。以总黄酮提取率为考察指标,在单因素试验的基础上,通过正交试验设计对桑白皮总黄酮提取工艺条件进行优化。在最佳提取工艺条件下,比较华南地区大面积种植的4个广东桑桑品种及采用3种取材方法获取的桑白皮的总黄酮提取率。优化的提取工艺条件为:乙醇体积分数60%,提取温度60℃,原料质量浓度25 g/L,浸提时间45 min,提取次数1次。桑品种及桑白皮的取材方法对总黄酮的提取率影响十分明显:同一桑品种用去木质部保留外表黄皮和整根切片2种方法获取的桑白皮中总黄酮提取率都明显大于除去外表黄皮及木质部取材的桑白皮;用去木质部保留外表黄皮和整根切片2种方法取材时,不同桑品种间桑白皮总黄酮的提取率差异较大,但用除去外表黄皮及木质部的方法取材时,不同桑品种间桑白皮总黄酮的提取率差异不明显。桑品种塘10和粤椹大10分别用去木质部保留外表黄皮及整根切片2种取材方法获取的桑白皮中总黄酮的含量较高,分别达到6.87、7.37 mg/g。
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| 关 键 词： | 桑白皮 总黄酮 提取工艺 取材方法 桑品种 |
Optimization on Extraction Technology of Total Flavonoids in Cortex Mori and Total Flavonoids Extraction Efficiencies of Various Methods |
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| LUO Guo-Qing,WU Yi-Ji,WANG Zhen-Jiang,LU Zhi-Jing,TANG Cui-Ming,SHEN Guang-Lin.Optimization on Extraction Technology of Total Flavonoids in Cortex Mori and Total Flavonoids Extraction Efficiencies of Various Methods[J].Acta Sericologica Sinica,2010,36(5):738-742. |
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| Authors: | LUO Guo-Qing WU Yi-Ji WANG Zhen-Jiang LU Zhi-Jing TANG Cui-Ming SHEN Guang-Lin |
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| Institution: | LUO Guo-Qing1 WU Yi-Ji2 WANG Zhen-Jiang1 LU Zhi-Jing2 TANG Cui-Ming1 SHEN Guang-Lin2(1The Sericulture & Farm Produce Processing Research Institute of Guangdong Academy of Agricultural Sciences,Guan-gzhou 510610,China,2Guangdong Branch of China Tobacco Industry Corporation,Guangzhou 510145,China) |
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| Abstract: | Flavonoids contained in cortex mori have various medicinal activities.In this study,single factor and orthogonal tests were used to optimize the extraction conditions for total flavonoids from cortex mori.Under the optimal extraction conditions,total flavonoids extraction rates from 4 Morus atropurpurea Roxb.varieties under commercial cultivation in South China regions and 3 different processing methods were compared.The optimum extraction conditions were as fol-lows: volume fraction of ethanol 60%,extracti... |
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| Keywords: | Cortex mori Total flavonoids Extraction technology Processing method Mulberry variety |
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