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| 条浒苔蛋白核超微结构和Rubisco及其活化酶分子定位 | |
| 引用本文： | 何培民.条浒苔蛋白核超微结构和Rubisco及其活化酶分子定位[J].水产学报,2004,28(3):255-260. |
| 作者姓名： | 何培民 |
| 作者单位： | 1. 上海水产大学生命科学与技术学院,上海,200090;南京农业大学农学院,南京,210095 2. 上海水产大学生命科学与技术学院,上海,200090 3. 南京农业大学农学院,南京,210095 4. 上海市农业科学院,上海,201106 5. 中国科学院上海植物生理研究所,上海,200433 |
| 基金项目： | 上海市曙光计划资助项目(SG-98-032)，国家自然科学基金资助项目(30371101) |
| 摘 要： | 研究了条浒苔蛋白核超微结构及其Rubisco和Rubisco活化酶金相免疫分子定位。超微结构显示,条浒苔细胞具有形态及组成相同的1～2个蛋白核,每个蛋白核被淀粉鞘所包围。蛋白核中央均有1条由1个类囊体构成的纵向孔道,并有时局部特别膨大。纵向孔道两端与叶绿体基质相连接。小球藻Rubisco抗体对条浒苔Rubisco的Western印迹图谱显示仅为1条带,其位置与SDS-PAGE电泳图谱上的主带相对应,分子量大约为55kD。金相免疫分子定位的结果显示,条浒苔Rubisco金标颗粒主要分布于叶绿体的蛋白核(71.86%)和淀粉鞘(27.94%)部位中,按面积密度计算二者总和占99.8%,极少分布在叶绿体类囊体和基质中(0.2%)。Rubisco活化酶分子定位也显示其主要分布于蛋白核和淀粉鞘中。这些结果均表明条浒苔蛋白核(及淀粉鞘)与单细胞绿藻的蛋白核相同,具有光合作用功能。条浒苔Rubisco初始活性和总活性的测定结果表明,其活化率较高,高达77.62%。 |
| 关 键 词： | 条浒苔 蛋白核 超微结构 Rubisco Rubisco活化酶 金相免疫分子定位 |
| 文章编号： | 1000-0615(2004)03-0255-06 |
| 收稿时间： | 2014/3/15 0:00:00 |
| 修稿时间： | 2003年1月16日 |
Pyrenoid ultrastructure and molecular localization of Rubisco and Rubisco activase in Enteromorpha clathrata |
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| HE Pei-min.Pyrenoid ultrastructure and molecular localization of Rubisco and Rubisco activase in Enteromorpha clathrata[J].Journal of Fisheries of China,2004,28(3):255-260. | |
| Authors: | HE Pei-min |
| Institution: | HE Pei-min~1,WU Qing-lei~1,WU Wei-ning~1,LU Wei~2,ZHANG Da-bing~3,CHEN Gen-yun~4,ZHANG Rong-xian~2 |
| Abstract: | The pyrenoid ultrastructure, goEnteromorpha clathrata were studied in this paper. E. clathrata is a marine macroalga with rapid growth and mutiple pyrenoids. The ultrastructure studies showed that there were 1-2 pyrenoids with similar shape and componE. clathrata. The pyrenoids were embedded by starch sheath. There was a portrait channel formed by a thylakoid through pyrenoid. Sometimes the portrait channel was enlarged in the middle of pyrenoid and was connected with the stroma of chloroplast at both channel ends. The Western blotting with antibody raised against Rubisco Chlorella protothecoides showed there was only one protein band in the Western blotting map. The SDS-PAGE and Western blotting sE. clathrata was about 55kDa. Gold immunolocalization showed the gold particles with antibodies raised against the large subunit of Rubisco heavily concentrated the pyrenoid (71.86%) and starch sheath (27.94%), both totalling 99.8% by area calculation, whereas the thylakoid region of the chloroplast was lightly labeled (0.2%). Rubisco activases also were mainly distributed in the pyrenoid and starch sheath. All the results demonstrated pyrenoid and starch sheath were closely related with photosynthetic function. Furthermore, the results of initial and total activity of Rubisco in E. clathrata determination of Rubisco showed its active ratio of Rubisco was as high as 77.62%. |
| Keywords: | Enteromorpha clathrata pyrenoid ultrastructure Rubisco Rubisco activase gold immunolocalization |
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