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Trypsin inhibitor in moth bean: thermal stability and changes during germination and cooking
Authors:S S Kadam  V M Ghorpade  R N Adsule  D K Salunkhe
Institution:(1) Department of Biochemistry, Mahatma Phule Agricultural University, 413 722 Rahuri, Maharashtra, India
Abstract:Trypsin inhibitor from moth bean was studied for thermal stability and changes during germination and cooking. The application of dry heat did not inactivate the inhibitor. However, autoclaving at 120°C at 15lbs pressure destroyed inhibitor activity completely. The extracted inhibitor lost 70% activity in 60 min when incubated at 100°C. Soaking of moth bean seeds for 8 h decreased trypsin inhibitor activity by 20%. The germination of seeds for 24 h resulted in 70% reduction in inhibitor activity. No activity was detected in 48 h germinated seeds. Germination (for 24 h) followed by cooking of moth bean seeds destroyed the trypsin inhibitor completely.
Keywords:Moth bean  trypsin inhibitor  thermal stability  cooking  germination
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