Random amplified polymorphic DNA (RAPD) variation in Fagopyrum tataricum Gaertn. accessions from China and the Himalayan region |
| |
| Authors: | TR Sharma S Jana |
| |
| Institution: | (1) Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, S7N 5A8, Canada |
| |
| Abstract: | The diversity among 52 landraces and cultivars of tartary buckwheat (Fagopyrum tataricum Gaertn.) and one accession of its wild ancestor, F. tataricum ssp. potanini Batalin, from diverse geographic origins was examined using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR)
markers. Eighteen primers produced a total of 240 fragments, of which 153 (63.75%) were monomorphic and 87 (36.25%) polymorphic
bands. UPGMA-based pairwise Jaccard’s coefficient of similarity was used to deduce the relationships among 53 genetically
diverse accessions. The similarity between cultivated tartary buckwheat accessions ranged from 0.61 to 1.00. Four distinct
clusters were formed which corresponded well with the geographic distribution of the tartary buckwheat. Nepalese accessions
showed maximum diversity followed by Chinese accessions. Tartary buckwheat accessions from the Himalayan region of northwestern
India revealed a narrow gene pool. The wild buckwheat accession did not group with any of the three cultivated tartary buckwheat
groups, and formed its own single-entry group. Genetic similarity (0.59) of Chinese buckwheat accessions with the wild ancestor
reaffirmed that cultivated tartary buckwheat originated in the Yunnan province of northwestern China. Consistent with some
earlier reports, our study demonstrated the usefulness of the RAPD technique for the characterization of plant genetic resources
and assessment of diversity between species.
This revised version was published online in August 2006 with corrections to the Cover Date. |
| |
| Keywords: | buckwheat Fagopyrum tataricum F tataricum ssp potanini RAPD diversity genetic variation |
| 本文献已被 SpringerLink 等数据库收录！ |
|
