| 地菍离体培养植株再生及其栽培试验 |
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| 引用本文： | 蒋道松,李玲,熊碧罗,李娈.地菍离体培养植株再生及其栽培试验[J].园艺学报,2007,34(3):787-790. |
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| 作者姓名： | 蒋道松 李玲 熊碧罗 李娈 |
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| 作者单位： | (湖南农业大学生物科学技术学院, 长沙410128; 长沙市园林管理局, 长沙410011) |
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| 摘 要： | 地菍叶片离体培养研究结果表明: 诱导愈伤组织最佳的培养基为MS + 1 mg·L-1 2, 4-D + 0.2mg·L-1 6-BA + 5%蔗糖+ 0.6%琼脂和MS + 2 mg·L-1 2, 4-D + 0.2 mg·L-1 6-BA + 5%蔗糖+ 0.6%琼脂; 丛生芽分化增殖最佳培养基为MS + 2 mg·L-1 6-BA + 0.3 mg·L-1 NAA + 5%蔗糖+ 0.6%琼脂; 生根最佳培养基为1/2MS + 1.5 mg·L-1NAA + 0.5 mg·L-1 2, 4-D + 5%蔗糖+ 0. 6%琼脂和MS + 0.5 mg·L-1 6-BA + 2mg·L -1 NAA + 5%蔗糖+ 0.6%琼脂。栽培观赏研究表明: 盆栽最佳温度为28～32℃; 最佳基质为泥炭土+蛭石(2 ∶1) 和腐殖土+蛭石(2 ∶1) ; 坪栽最佳肥料配比为N ∶P ∶K = 114 ∶112 ∶1, 最佳土壤为腐殖土。
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| 关 键 词： | 地菍 离体培养 植株再生 栽培 观赏植物 |
| 文章编号： | 0513-353X（2007）03-0787-04 |
| 修稿时间： | 2006-11-062007-04-02 |
Research on in Vitro Culture for Plant Regeneration and Cultivation of Ornamental of Melastoma dodecandrum |
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| JIANG Dao-song,LI Ling,XIONG Bi-luo,LI Luan.Research on in Vitro Culture for Plant Regeneration and Cultivation of Ornamental of Melastoma dodecandrum[J].Acta Horticulturae Sinica,2007,34(3):787-790. |
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| Authors: | JIANG Dao-song LI Ling XIONG Bi-luo LI Luan |
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| Institution: | (;1. College of Bioscience and Technology, Hunan Agricultural University, Changsha 410128, China;2.Garden Management Bureauof Changsha City, Changsha 410007, China) |
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| Abstract: | In vitro culture and plant regeneration of Melastoma dodecandrum by tissue culture using itsblade were studied. The results showed that the op timum medium for callus induction isMS supp lemented with2, 4-D 1 mg·L -1 , 6-BA 0.2 mg·L-1 , 5% sucrose and 0.6% agar , or MS with 2, 4-D 2 mg·L-1 , 6-BA 0.2 mg·L -1 , 5% sucrose and 0.6% agar. The optimum medium for the formation and growth of clusterbuds is MS with 6-BA 2 mg·L-1 , NAA 0.3 mg·L-1 , 5% sucrose and 0.6% agar. The optimum mediumfor rooting is 1/2MS with NAA 1.5 mg·L-1 , 2, 4-D 0.5 mg·L-1 , 5% sucrose and 0.6% agar, or MSwith 6-BA 0.5 mg·L-1 , NAA 2 mg·L-1 , 5% sucrose and 0.6% agar. The cultivation for ornamental oftest-tube plantlets was also studied. The optimum temperature is 28 - 32℃. The optimum medium is 2/3 peatsoil and 1/3 vermiculite, or 2/3 humus and 1/3 vermiculite for pot cultivation. The best p roportion of N, Pand K is 114 ∶112 ∶1. The optimum soil is humus. The test2tube p lantlets can be cultivated as a pot flowerand ornamental lawn with high ornamental value. |
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| Keywords: | Melastoma dodecandrum In vitro culture Regeneration Cultivation Ornamental |
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