| 利用叶片再生进行滁菊组培扩繁 |
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| 引用本文： | 龚建国,杨雪,李婷,牛毅,范加勤.利用叶片再生进行滁菊组培扩繁[J].安徽农业科学,2013(18):7788-7789. |
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| 作者姓名： | 龚建国 杨雪 李婷 牛毅 范加勤 |
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| 作者单位： | 安徽省滁州市滁菊研究所,安徽滁州,239000;安徽省农业科学院,安徽合肥230031;南京农业大学植物保护学院,江苏南京210095;南京农业大学植物保护学院,江苏南京,210095 |
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| 基金项目： | 安徽省委组织部"115"产业创新团队建设项目 |
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| 摘 要： | 目的]滁菊高效快速繁殖体系的建立。方法]以叶片为外植体,选用不同培养基并添加激素6-BA和NAA,研究不同激素浓度组合培养基对外植体愈伤诱导、不定芽分化及生根的影响,获得选择分化率高的培养条件。结果]滁菊叶片最适增殖和分化培养基分别为MS+6-BA 2 mg/L+NAA 0.1 mg/L和MS+6-BA 0.5 mg/L+NAA 0.1 mg/L,最佳生根培养基为1/2 MS+NAA 0.2 mg/L,生根率达100%。结论]该研究为滁菊的快速繁殖提供了技术依据。
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| 关 键 词： | 滁菊 叶片外植体 分化 生根 移栽 |
Multiplication of Dendranthema morifolium (Ramat.) Tzvel cv.Chuju through Leaf Cell Regenerating |
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| Institution: | GONG Jian-guo( 1.Chuzhou Institute of Chuju, Chuzhou, Anhui 239000;) |
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| Abstract: | Objective] To establish a rapid and efficient propagation system for Chuju.Method] With leaf as explants,selecting different media added with 6-BA and NAA,effects of different concentration hormone on callus induction,differentiation of adventitious bud sand rooting were studied,an optimum condition for high multiplication rate from Chuju leaves was obtained.Result] 6-BA 2 mg/L and NAA 0.1mg/L added to MS and 6-BA 0.5 mg/L and NAA 0.1 mg/L added to MS were better for proliferating and regenerating from Chuju leaves respectively,and half MS supplement with NAA 0.2 mg/L was good for rooting,the rooting rate was 100 percent.Conclusion] The study could provide technique basis for rapid micropropagation of Chuju. |
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| Keywords: | Dendranthema morifolium (Ramat ) Tzvel cv Chuju Leaf explants Differentiation Rooting Transplanting |
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