| 产苯乳酸乳酸菌的筛选及鉴定 |
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| 引用本文： | 张莉力,柴虹宇.产苯乳酸乳酸菌的筛选及鉴定[J].安徽农业科学,2010,38(5):2590-2592. |
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| 作者姓名： | 张莉力 柴虹宇 |
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| 作者单位： | 辽宁医学院食品科学与工程学院,辽宁锦州,121001 |
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| 基金项目： | 辽宁省教育厅资助项目(2008431) |
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| 摘 要： | 目的]研究产苯乳酸乳酸菌的筛选与鉴定。方法]用双层培养基法从传统发酵制品中分离出115株乳酸菌,用双层平板法从中筛选出24株对啤酒酵母具有明显抑菌效果的菌株,然后通过高效液相色谱分析筛选产苯乳酸量最高的菌株,并对筛选的菌株进行形态学观察、生理生化鉴定以及菌株16S rDNA序列的测定与分析。结果]对初筛的24株菌株发酵液进行高效液相色谱分析得到7株苯乳酸的产量〉60 mg/L的菌株,其中菌株P421乳酸菌显示出最高的苯乳酸生产能力,苯乳酸产量可达80.5 mg/L。根据菌株P421的形态特征、生理生化特征和16S rDNA序列分析,将该菌株鉴定为植物乳杆菌（Lactobacillus plantarum）。结论]从传统发酵制品中分离筛选得到1株产苯乳酸的菌株,建立了1种准确筛选苯乳酸生产菌的方法。
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| 关 键 词： | 苯乳酸 乳酸菌 鉴定 生物防腐 |
Screening and Identification of Lactic Acid Bacteria Producing Phenyllactic Acid |
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| ZHANG Li-li et al.Screening and Identification of Lactic Acid Bacteria Producing Phenyllactic Acid[J].Journal of Anhui Agricultural Sciences,2010,38(5):2590-2592. |
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| Authors: | ZHANG Li-li |
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| Institution: | College of Food Science and Engineering;Liaoning Medical University;Jinzhou;Liaoning 121001 |
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| Abstract: | Objective] The study aimed to research the screening and identification of lactic acid bacteria producing phenyllactic acid.Method] The 115 lactic acid bacteria strains were separated from the traditional fermented products by using double-medium method.Among them,the 24 strains which had obvious bacteriostasis effect on beer yeast were screened by using double-layer plate method,and then the strain that could produce highest phenyllactic acid was screened and analyzed through HPLC method.The screened str... |
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| Keywords: | Phenyllactic acid Lactic acid bacteria Identification Biopreservation |
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