| 海南番木瓜花叶病毒全长cDNA克隆及序列分析 |
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| 引用本文： | 王永辰,沈文涛,王树昌,庹德财,言普,黎小瑛,周鹏.海南番木瓜花叶病毒全长cDNA克隆及序列分析[J].热带作物学报,2013,34(2):297-300. |
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| 作者姓名： | 王永辰 沈文涛 王树昌 庹德财 言普 黎小瑛 周鹏 |
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| 作者单位： | 1. 海南大学农学院,海南海口 570228;中国热带农业科学院热带生物技术研究所,海南海口 571101
2. 中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 基金项目： | 国家自然科学基金项目（No. 30560082、31000844）；海南省重点科技计划（No. ZDXM20120027）。 |
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| 摘 要： | 提取海南果园番木瓜病叶样品总RNA,反转录得到cDNA第一链,基于已报道的番木瓜花叶病毒(Papaya mosaic virus,PaMV)全长基因组序列,设计引物,PCR扩增PaMV-CP基因序列,通过测序和序列比对分析确定发病植物感染了PaMV.再通过RT-PCR扩增PaMV全长cDNA序列,克隆及测序结果表明,所获得的cDNA全长为6656 bp,与国外报道的PaMV分离物全长核苷酸序列的相似性达99.6％.
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| 关 键 词： | 番木瓜花叶病毒 全长cDNA 克隆 序列分析 |
Complete Genomic Sequence of Papaya mosaic virus Isolate from Hainan Island, China |
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| WANG Yongchen,SHEN Wentao,WANG Shuchang,TUO Decai,YAN Pu,LI Xiaoying and ZHOU Peng.Complete Genomic Sequence of Papaya mosaic virus Isolate from Hainan Island, China[J].Chinese Journal of Tropical Crops,2013,34(2):297-300. |
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| Authors: | WANG Yongchen SHEN Wentao WANG Shuchang TUO Decai YAN Pu LI Xiaoying and ZHOU Peng |
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| Institution: | 1 Agriculture College, Hainan University
2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 Agriculture College, Hainan University
2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 Agriculture College, Hainan University
2 Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences |
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| Abstract: | |
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| Keywords: | PaMV Full-length cDNA Cloning Sequece analysis |
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