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长期施药果园中的苹果轮纹病菌对戊唑醇和甲基硫菌灵的敏感性
引用本文:安久栋,国立耘,朱小琼,宋月凤.长期施药果园中的苹果轮纹病菌对戊唑醇和甲基硫菌灵的敏感性[J].植物保护,2016,42(2):187-191.
作者姓名:安久栋  国立耘  朱小琼  宋月凤
作者单位:中国农业大学植物保护学院,北京,100193
基金项目:国家现代农业产业技术体系(CARS-28)
摘    要:为了监测果园中苹果轮纹病菌对戊唑醇和甲基硫菌灵敏感水平的变化,采用菌丝生长速率法测定了采集自山东烟台地区和北京市昌平区,有较长施药史苹果园中的苹果轮纹病菌对戊唑醇和甲基硫菌灵的敏感性。结果表明:戊唑醇对连续施用戊唑醇5年、每年施药1~2次的果园中苹果轮纹病菌的EC_(50)为0.017 4~0.114 3μg/mL,即该类果园中苹果轮纹病菌对此药的敏感性仍然保持在较高水平,与野生菌株的敏感性非常接近,没有出现敏感性分化的抗药亚群体;甲基硫菌灵对连续施用甲基硫菌灵10年、每年施药2次的果园中苹果轮纹病菌的EC_(50)为0.846 4~4.677 4μg/mL,果园中苹果轮纹病菌与野生菌株相比EC_(50)平均值约上升3.15倍,最低值和最高值分别是已报道敏感性基线的1.19倍和6.59倍,没有出现敏感性发生显著分化的抗药性亚群体。

关 键 词:苹果轮纹病菌  戊唑醇  甲基硫菌灵  敏感性
收稿时间:1/8/2015 12:00:00 AM
修稿时间:6/8/2015 12:00:00 AM

Susceptibility of Botryosphaeria dothidea to tebuconazole and thiophanate-methyl
An Jiudong,Guo Liyun,Zhu Xiaoqiong,Song Yuefeng.Susceptibility of Botryosphaeria dothidea to tebuconazole and thiophanate-methyl[J].Plant Protection,2016,42(2):187-191.
Authors:An Jiudong  Guo Liyun  Zhu Xiaoqiong  Song Yuefeng
Institution:College of Plant Protection, China Agricultural University, Beijing 100193, China
Abstract:In order to monitor the susceptibility of Botryosphaeria dothidea to tebuconazole and thiophanate-meth-yl in orchards with long history of using these two fungicides,the susceptibility of B .dothidea isolates collected from represented orchards to these two fungicides was determined based on mycelium growth rate.The results showed that the EC50 values of tebuconazole against 69 isolates collected from Yantai,Shandong Province with ap-plication of tebuconazole for 1-2 times each year for 5 years ranged from 0.017 4 to 0.114 3 μg/mL with no ap-peared resistant sub-populations.The EC50 values of thiophanate-methyl against 34 isolates collected from Chang-ping District,Beijing with application of thiophanate-methyl for 2 times each year for 10 years ranged from 0.846 4 to 4.677 4 μg/mL with no appeared resistant sub-populations.The maximum and minimum values were 1.19 and 6.59 times of the reported susceptible baseline,respectively.
Keywords:Botryosphaeria dothidea  tebuconazole  thiophanate-methyl  susceptibility
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