| 香蕉NBS-LRR类抗病基因同源序列的克隆与分析 |
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| 引用本文： | 袁克华,冯仁军,程 萍,张银东.香蕉NBS-LRR类抗病基因同源序列的克隆与分析[J].中国农学通报,2009,25(5):271-274. |
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| 作者姓名： | 袁克华 冯仁军 程 萍 张银东 |
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| 作者单位： | 海南大学,海口,570228;中国热带农业科学院热带生物技术研究所,海口,571101 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金资助项目 |
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| 摘 要： | 根据已知植物抗病基因的保守区域设计引物,从香蕉基因组DNA扩增出一条与植物抗病基因同源的序列,命名为BRGA1,该同源片段含有典型的NBS-LRR类抗病基因所拥有的保守性结构p-loop、Kinase-2a、Kinase-3a和疏水结构域HD,它与部分已知NBS-LRR类抗病基因的氨基酸序列同源性为23.8%-42.7%。Northern杂交表明BRGA1在香蕉中受水杨酸调控,属诱导型表达。
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| 关 键 词： | 香蕉NBS—LRR 同源克隆技术 抗病基因同源序列 水杨酸 |
| 收稿时间： | 2008-12-05 |
| 修稿时间： | 2008-12-26 |
Cloning and Identification of the Resistance Gene Analogs of Banana |
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| Yuan Kehua,Feng Renjun,Cheng Ping,Zhang Yindong.Cloning and Identification of the Resistance Gene Analogs of Banana[J].Chinese Agricultural Science Bulletin,2009,25(5):271-274. |
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| Authors: | Yuan Kehua Feng Renjun Cheng Ping Zhang Yindong |
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| Institution: | Yuan Kehua, Feng Renjun, Cheng Ping, Zhang Yindong (1.College of Agronomy, HainanUniversity, Haikou 570228; 2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101) |
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| Abstract: | Specific primer sequences were designed on the basis of converse motifs of cloned resistance genes and subsequently used as PCR primers to amplify the resistance gene analog(RGA) in banana.The amplified product of BRGA1 was cloned and sequenced.The banana RGA contain some conserved motifs such as p-loop,Kinase-2a,Kinase-3a and HD presenting in known NBS-LRR resistance genes from other plants and share between 23.8%-42.7% amino acid sequence identity with them.The BRGA1 expression was induced and detected by Northern blotting,which is positively regulated by salicylic acid. |
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| Keywords: | banana NBS-LRR homology-based cloning resistance gene analog （RGA） salicylic acid |
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