Characterization of a metalloproteinase component extracted from soil |
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| Authors: | K Hayano M Takeuchi E Ichishima |
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| Institution: | (1) National Institute of Agro-environmental Sciences, Yatabe, Tsukuba, 305 Ibaraki, Japan;(2) Tokyo Nôkô University, 183 Fuchu, Tokyo, Japan;(3) Present address: Laboratory of Soil Microbiology, Kyushu Agricultural Experiment Station, Ministry of Agriculture, Forestry and Fisheries, 833 Chikugo, Fukuoka, Japan |
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| Abstract: | Summary A metalloproteinase was found to be the main component of a protease in the extract from an Andosol collected from a tomato field. The protease has a pH optimum of 7 for benzyloxycarbonyl-L-phenylalanyl-L-tyrosyl-L-leucine with tyrosylleucine as the main reaction product. The Km value for the substrate was 0.4 mM. Activity was inhibited by EDTA but not by pepstatin, p-chloromercuribenzoate and phenylmethanesulfonyl fluoride. After the removal of EDTA from the inactivated enzyme by dialysis and the addition of metal ions (Zn2+, Mn2+ and Fe3+), the enzyme activity could be recovered. The apparent isoelectric points of the metalloproteinase components were estimated to be 4.9, 4.5 and 4.1 by isoelectric focusing. A fraction with an apparent isoelectric point of 4.9 was the main component. The apparent molecular weight of the main protease component was estimated to be 4.7 × 104 by gel filtration of Sephadex G-100. The enzyme hydrolyzed a natural polypeptide, angiotensin I (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu). Main split sites in the peptide were -Tyr7-Ile5- and -Pro7-Phe8-. The former was the most sensitive site to the soil metalloproteinase concerned. |
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| Keywords: | Proteinases Metalloproteinase Soil enzyme Soil extract Nitrogen transformation |
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