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| 利用鳕鱼皮制备饲用胶原肽添加剂及其抗氧化研究 | |
| 引用本文： | 刘峰,刘春娥,李亚,李志凌,许乐乐,李梦龙,杨烨.利用鳕鱼皮制备饲用胶原肽添加剂及其抗氧化研究[J].饲料工业,2011,32(22). |
| 作者姓名： | 刘峰 刘春娥 李亚 李志凌 许乐乐 李梦龙 杨烨 |
| 作者单位： | 1. 中国农业大学烟台研究院,264670,山东烟台 2. 烟台嘉惠海洋生物科技有限公司 3. 山东东方海洋科技股份有限公司 |
| 基金项目： | 烟台市科技发展计划项目 |
| 摘 要： | 以鳕鱼皮为原料,采用双酶复合法提取胶原多肽作为饲用添加剂。以水解度为指标,通过正交试验确定酶解的最适条件。结果表明:碱性蛋白酶和木瓜蛋白酶混合水解,加酶量分别为4%和4.5%,温度55℃p、H值8,时间11 h,蛋白质水解度可达到25.61%。分离得到的多肽组分,Mr<3 500 Da的占82%以上,抗氧化试验结果表明,水解产物有较好的抗氧化特性,有望作为高档水产饲料添加剂使用。 |
| 关 键 词： | 鳕鱼皮 胶原肽 饲料添加剂 抗氧化 |
Study on the extraction and antioxidant activity of the collagen peptides for feed additive from cod skin |
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| Liu Feng , Liu Chun'e , Li Ya , Li Zhiling , Xu Lele , Li Menglong , Yang Ye.Study on the extraction and antioxidant activity of the collagen peptides for feed additive from cod skin[J].Feed Industry,2011,32(22). | |
| Authors: | Liu Feng Liu Chun'e Li Ya Li Zhiling Xu Lele Li Menglong Yang Ye |
| Abstract: | The research studied the preparation of peptide from cod skin for feed additive by compound enzyme hydrolysis.The optimal reaction conditions were determined by orthogonal experiments with the hydrolysis for index.The results showed that the optimal addition amount of alcalase and papain was 4%,and 4.5%respectively,the enzymatic hydrolysis temperature was 55 ℃,pH value was 8 and hydrolysis time was 11 h.Under the above conditions,the extraction yield of collagen peptides from cod skin was 25.61%.Among the hydrolysates,yield of Mr<3 500 Da was over 82%.Antioxidant activity of the product was good enough to be immunostimulation of fish. |
| Keywords: | cod skin collagen peptide feed additive antioxygen |
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