| 一个水稻开颖不育突变体ohs1(t)的遗传分析及基因定位 |
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| 引用本文： | 刘晓玲,毛毕刚,刘华清,陈建民,田大刚,王锋.一个水稻开颖不育突变体ohs1(t)的遗传分析及基因定位[J].分子植物育种,2009,7(2). |
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| 作者姓名： | 刘晓玲 毛毕刚 刘华清 陈建民 田大刚 王锋 |
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| 作者单位： | 1. 福建省农业科学院生物技术研究所,福建省农业遗传工程重点实验室,福州,350003;福建师范大学生命科学学院,福州,350007
2. 福建省农业科学院生物技术研究所,福建省农业遗传工程重点实验室,福州,350003 |
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| 摘 要： | 我们在明恢86的转基因后代中发现了一个水稻花器官发育突变体,暂命名为开颖不育(open hull sterile 1,ohs1(t)))突变体.ohs1(t)突变体表现颖花开裂,内外稃片变细,内稃微弯向外稃,有雌雄蕊分化,但雌雄蕊较野生犁株的小,大多数花药没有花粉,少数花药中含有不育花粉,雌雄配子均不育.突变体ohs1(t)分别与明恢86、R527、93-11和中花16号杂交后代遗传分析表明,ohs1(t)是一个隐性基因控制的突变体.以ohs1(t)和93-11杂交F2群体中突变个体作为初步定位群体,采用已报道的SSR标记将OHS1(t)初步定位在1号染色体的长臂端RM493和RM5638两个标记间.随后利用已公布的水稻基因组序列(http://rgp.dna.affrc.go.jp/E/index.html)及93-11和日本晴间SSR标记数据库(http://www.gramene.org/),新开发和筛选了SSR和InDel标记,并以ohs1(t))和中花16号杂交F2群体中突变个体作为新定位群体,将OHS1(t)基因进一步定位在NSSR0115和InDel0102之间,遗传距离分别为0.2 cM和0.3 cM,物理距离约66 kb.
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| 关 键 词： | 水稻 开颖不育突变体 遗传分析 基因定位 |
Genetic Analysis and Gene Mapping of ohs1(t), an Open Hull Sterile Mutant in Rice (Oryza Sativa L.) |
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| Liu Xiaoling,Mao Bigang,Liu Huaqing,Chen Jianmin,Tian Dagang,Wang Feng.Genetic Analysis and Gene Mapping of ohs1(t), an Open Hull Sterile Mutant in Rice (Oryza Sativa L.)[J].Molecular Plant Breeding,2009,7(2). |
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| Authors: | Liu Xiaoling Mao Bigang Liu Huaqing Chen Jianmin Tian Dagang Wang Feng |
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| Institution: | Liu Xiaoling 1,2 Mao Bigang 1 Liu Huaqing 1 Chen Jianmin 1 Tian Dagang 1 Wang Feng 1,2 1 Institute of Biotechnology,Fujian Academy of Agricultural Sciences,Fujian Key Laboratory of Genetic Engineering for Agriculture,Fuzhou,350003,2 College of Life Sciences,Fujian Normal University,350007 |
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| Abstract: | We screened a flower development mutant in the transgenic progenies of indica rice Minghui 86, which is tentatively named open hull sterile 1 (ohs1(t)). The mutant ohs1(t) was characterised by open-hull sterility, smaller and abnormal lemma and palea. There were structure of the anthers and pistil in the mutant, but the anthers and pistil of the mutant were white and small, non-pollen or without viable pollen. Female and male gametes were sterile in the mutant. Genetic analysis of the F1 and F2 populations ... |
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