| 健脾益肠散对溃疡性结肠炎大鼠CD86和ICAM-1表达的影响 |
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| 作者姓名： | 蔺晓源 徐菲 张嘉伦 赵应松 王敏 刘杰民 胡国恒 |
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| 作者单位： | 湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学中医学国内一流建设学科, 湖南 长沙 410208,贵阳中医学院, 贵州 贵阳 550002,贵阳中医学院, 贵州 贵阳 550002,贵阳中医学院, 贵州 贵阳 550002,贵阳中医学院, 贵州 贵阳 550002,贵州省人民医院, 贵州 贵阳 550002,湖南中医药大学第一附属医院, 湖南 长沙 410007 |
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| 基金项目： | 国家自然科学基金项目（81560773）。 |
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| 摘 要： | 目的 观察健脾益肠散对溃疡性结肠炎（ulcerative colitis，UC）大鼠CD86分子（CD86）和细胞间黏附分子-1（intercellular adhesion molecule-1，ICAM-1）表达的影响，探讨其对UC肠黏膜的免疫保护机制。方法 将60只SD大鼠先随机分为空白组和造模组，造模组采用2，4，6-三硝基苯磺酸（2，4，6-trinitrobenzene sulfonic acid，TNBS）溶液灌肠法制备UC大鼠模型，待模型复制成功后再将造模组随机分为模型组、西药组和中药高、中、低剂量组；干预21 d后，免疫组化法检测各组大鼠结肠组织中CD86的阳性表达，Western Blot、RT-PCR法分别检测结肠中ICAM-1的蛋白和mRNA表达。结果 与空白组比较，模型组大鼠结肠组织中CD86的阳性表达以及ICAM-1的蛋白和mRNA表达均显著增高（P<0.01）；与模型组比较，中药高、中、低剂量组和西药组CD86、ICAM-1的表达均不同程度地降低（P<0.05或P<0.01），且以中药高剂量组最为明显（P<0.05或P<0.01）。结论 健脾益肠散可能通过降低结肠组织中CD86、ICAM-1的表达水平起到对UC结肠黏膜的免疫保护作用。
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| 关 键 词： | 溃疡性结肠炎 健脾益肠散 CD86分子 细胞间黏附分子-1 |
| 收稿时间： | 2018/12/4 0:00:00 |
Effects of Jianpi Yichang Powder on Expression of CD86 Molecule, Intercellular Adhesion Molecule-1 in Rats with Ulcerative Colitis |
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| Authors: | LIN Xiaoyuan XU Fei ZHANG Jialun ZHAO Yingsong WANG Min LIU Jiemin and HU Guoheng |
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| Institution: | Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;The Domestic First Class Construction Discipline of Chinese Medicine in Hunan University of Chinese Medicine, Changsha, Hunan 410208, China,Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550002, China,Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550002, China,Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550002, China,Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou 550002, China,People''s Hospital of Guizhou Province, Guiyang, Guizhou 550002, China and The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China |
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| Abstract: | Objective To observe the effects of Jianpi Yichang Powder (JYP) on the expressions of CD86 molecule (CD86) and intercellular adhesion molecule-1 (ICAM-1) in ulcerative colitis (UC) rats, and to explore its immunoprotective mechanism of UC intestinal mucosa. Methods A total of 60 SD rats were randomly divided into a blank group and a modeling group. The UC models were established by the method of 2,4,6-trinitrobenzene sulfonic acid (TNBS) solution clyster. Then the successfully modeled rats were randomly divided into a model group, a western medicine group, a high, middle, and low dosage groups of Chinese herbal medicine. After intragastric administration for 21 days, immunohistochemical method was used to detect the positive expression of CD86. Western Blot and Real-Time PCR methods were used to detect the expression of ICAM-1 protein and mRNA in colonic tissue of rats in each group. Results The positive expression of CD86 and the protein and mRNA expressions of ICAM-1 in colonic tissue of the model group were significantly increased than those in the blank group (P<0.01). The expressions of CD86 and ICAM-1 in high, middle, low dose groups of Chinese herbal medicine groups and western medicine groups were decreased than the model group in different degrees (P<0.05 or P<0.01), and the high dose group of Chinese herbal medicine was the most obvious (P<0.05 or P<0.01). Conclusion JYP may play an immune protective role on colon mucosa of UC by decreasing the expression of CD86, ICAM-1 in colon tissue. |
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| Keywords: | ulcerative colitis Jianpi Yichang Powder CD86 molecule intercellular adhesion molecule-1 |
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