| 61个小麦后备品种对白粉病的抗性分析及抗病基因推导 |
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| 引用本文： | 史文琦,龚双军,曾凡松,向礼波,杨立军,喻大昭.61个小麦后备品种对白粉病的抗性分析及抗病基因推导[J].植物保护学报,2019,46(5):1086-1099. |
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| 作者姓名： | 史文琦 龚双军 曾凡松 向礼波 杨立军 喻大昭 |
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| 作者单位： | 湖北省农业科学院植保土肥研究所, 农业部华中作物有害生物综合治理重点实验室, 农作物重大病虫草害防控湖北省重点实验室, 武汉 430064,湖北省农业科学院植保土肥研究所, 农业部华中作物有害生物综合治理重点实验室, 农作物重大病虫草害防控湖北省重点实验室, 武汉 430064,湖北省农业科学院植保土肥研究所, 农业部华中作物有害生物综合治理重点实验室, 农作物重大病虫草害防控湖北省重点实验室, 武汉 430064,湖北省农业科学院植保土肥研究所, 农业部华中作物有害生物综合治理重点实验室, 农作物重大病虫草害防控湖北省重点实验室, 武汉 430064,湖北省农业科学院植保土肥研究所, 农业部华中作物有害生物综合治理重点实验室, 农作物重大病虫草害防控湖北省重点实验室, 武汉 430064,湖北省农业科学院植保土肥研究所, 农业部华中作物有害生物综合治理重点实验室, 农作物重大病虫草害防控湖北省重点实验室, 武汉 430064 |
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| 基金项目： | 国家重点研发计划（2016YFD0300700），国家自然科学基金（31701749），国家现代农业（小麦）产业技术体系（CARS-03-05B） |
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| 摘 要： | 为明确中国61个小麦后备品种对白粉病的抗性水平及其抗病基因,将2016年从黄淮海冬麦区、长江中下游冬麦区及东北春麦区的9个市采集分离的269株单孢子堆白粉病菌,分别接种于61个小麦后备品种进行抗性测定;用NTSYSpc 2.10e软件对供试品种表型数据进行聚类分析;用35株鉴别菌株对29个含已知抗白粉病基因小麦材料和61个小麦后备品种进行鉴别,比较其抗谱并推导61个小麦后备品种所含抗白粉病基因。结果显示,61个小麦后备品种间的抗谱存在明显差异,国豪麦5号和7号、BL5008、绵麦系列、黔麦系列、楚麦16号、内麦101和366等18个品种抗谱较宽,抗性频率均大于97.0%;泰科麦5303、邯11-5272和临Y8222等10个品种的抗性频率在42.0%～56.1%之间;郑麦0943等33个品种的抗性频率小于37.9%。聚类分析可将61个小麦后备品种分成5大类,第I类有11个品种,其中8个品种的抗性频率在42.0%～56.1%之间;第II类和第III类共30个品种,抗性频率均小于32.7%;第IV类有2个品种,抗性频率分别为53.5%和53.2%,第V类有18个品种,抗性频率均大于97.0%;聚类显示来自于同一地区且抗性频率相近的品种具有相似或相近的抗性遗传背景。本研究推导出21个小麦后备品种含抗病Pm基因,其中,邯11-5272含有Pm30,安科1503含有Pm2、Pm5a、Pm6、Pm19和Pm30,临Y8222含有Pm5a、Pm6、Pm19和Pm30,云154-15含有Pm5a、Pm6、Pm7、Pm19和Pm2+ta,泰科麦5303等6个品种含有Pm2和Pm30,华麦7号等5个品种含有Pm5a、Pm6和Pm19,扬麦24号等6个品种含有Pm5a、Pm6、Pm19和Pm2+ta。研究表明,54.1%的小麦后备品种对白粉病菌群的抗性频率小于37.9%,存在适宜条件下小麦白粉病暴发流行的风险,因此这些小麦后备品种推广种植时需加强病害预警和监测。
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| 关 键 词： | 小麦白粉病 抗性频率 抗病基因 基因推导 |
| 收稿时间： | 2018/10/31 0:00:00 |
Evaluation of resistance to powdery mildew in 61 Chinese wheat cultivars and postulation of their resistance genes |
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| Shi Wenqi,Gong Shuangjun,Zeng Fansong,Xiang Libo,Yang Lijun and Yu Dazhao.Evaluation of resistance to powdery mildew in 61 Chinese wheat cultivars and postulation of their resistance genes[J].Acta Phytophylacica Sinica,2019,46(5):1086-1099. |
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| Authors: | Shi Wenqi Gong Shuangjun Zeng Fansong Xiang Libo Yang Lijun and Yu Dazhao |
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| Institution: | Hubei Key Laboratory of Crop Diseases, Insect Pests and Corp Weeds Control;Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture;Institute for Plant Protection and Soil Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei Province, China,Hubei Key Laboratory of Crop Diseases, Insect Pests and Corp Weeds Control;Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture;Institute for Plant Protection and Soil Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei Province, China,Hubei Key Laboratory of Crop Diseases, Insect Pests and Corp Weeds Control;Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture;Institute for Plant Protection and Soil Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei Province, China,Hubei Key Laboratory of Crop Diseases, Insect Pests and Corp Weeds Control;Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture;Institute for Plant Protection and Soil Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei Province, China,Hubei Key Laboratory of Crop Diseases, Insect Pests and Corp Weeds Control;Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture;Institute for Plant Protection and Soil Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei Province, China and Hubei Key Laboratory of Crop Diseases, Insect Pests and Corp Weeds Control;Key Laboratory of Integrated Pest Management on Crops in Central China, Ministry of Agriculture;Institute for Plant Protection and Soil Sciences, Hubei Academy of Agricultural Sciences, Wuhan 430064, Hubei Province, China |
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| Abstract: | To analyze the resistance of 61 wheat cultivars and postulate their resistance genes to powdery mildew, 269 wheat single colony of powdery mildew isolates were collected from nine cities which grew wheat in China in 2016, the resistance frequencies of 61 wheat cultivars were estimated by the mean of inoculation detached leaves segments of each cultivar with 269 isolates, respectively. Cluster analysis was carried out with UPGMA (unweighted pair group method with arithmetic mean) model of the software NTSYSpc 2.1e based on the phenotypic resistance data. And resistance genes of the 61 cultivars were postulated by comparing resistance spectra of the cultivars to 35 differential isolates with those of materials possessing known resistance genes to these isolates. The results showed that the resistance spectrum of each cultivar differed significantly from that of others, the resistance frequency of 18 wheat cultivars such as Guohaomai No. 5 and No. 7, BL5008, series of Mianmai and series of Qianmai, Chumai No. 16, Neimai 101 and 366 were higher than 97.0%, while the resistance frequency of ten wheat cultivars such as Taikemai 5303, Han 11-5272, Lin Y8222 and so on, were between 42.0% and 56.1%. Among the 61 cultivars, 33 cultivars such as Zhengmai 0943 had resistance frequency lower than 37.9%. Cluster analysis indicated 61 cultivars could be classified into five groups. Group one included 11 cultivars and out of them, resistance frequency of eight cultivars to all isolates was between 42.0% and 56.1%. Groups two and three including 30 cultivars with resistance frequency lower than 32.7%. Group four including two cultivars with resistance frequency 53.5% and 53.2%, while the fifth group composed of 18 cultivars with resistance frequency higher than 97.0%. The cultivars come from the same cities and with the similar frequency of resistance had been clustered to the same group. It showed that they might have a similar genetic background of resistance. Results of Pm gene postulation showed that Han 11-5272 contained Pm30, Anke 1503 possessed Pm2, Pm5a, Pm6, Pm19 and Pm30, Lin Y8222 contained Pm5a, Pm6, Pm19 and Pm30, Yun 154-15 contained Pm5a, Pm6, Pm7, Pm19 and Pm2+ta, Taikemai 5303 and other five cultivars possessed Pm2 and Pm30, Huamai No. 7 and other four cultivars possessed Pm5a, Pm6 and Pm19, Yangmai No. 24 and other five cultivars contained Pm5a, Pm6, Pm19 and Pm2+ta. It indicated that 33 cultivars among the 61 cultivars, counting for 54.1%, had frequencies of resistance lower than 37.9%, suggesting the epidemic risk of wheat powdery mildew. Those cultivars should be monitored strictly when they are planted in epidemic regions. |
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| Keywords: | wheat powdery mildew resistance frequency resistance gene gene postulation |
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