| 鲁米诺化学发光测定精胺 |
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| 引用本文： | 吴秋华,李正平,马晶军,冯硕,方正.鲁米诺化学发光测定精胺[J].河北农业大学学报,2004,27(6):112-115. |
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| 作者姓名： | 吴秋华 李正平 马晶军 冯硕 方正 |
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| 作者单位： | 1. 河北农业大学,生物无机化学重点实验室,河北,保定,071001
2. 河北农业大学,生物无机化学重点实验室,河北,保定,071001;河北大学,化学与环境科学学院,河北,保定,071001 |
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| 摘 要： | 铜与精胺形成的1∶1不饱和配合物对Luminol-H2O2化学发光体系有强烈的催化活性,据此建立了简便、灵敏的测定精胺的化学发光新方法,线性范围为10-7～10-5mol/L,检测限为4×10-9mol/L,相对标准偏差为5 55%。
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| 关 键 词： | 鲁米诺 化学发光 精胺 铜 |
| 文章编号： | 1000-1573(2004)06-0112-04 |
Determination of spermine by luminol chemiluminescence determination |
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| WU Qiu-hua,LI Zheng-ping.Determination of spermine by luminol chemiluminescence determination[J].Journal of Agricultural University of Hebei,2004,27(6):112-115. |
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| Authors: | WU Qiu-hua LI Zheng-ping |
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| Institution: | WU Qiu-hua~1,LI Zheng-ping~ |
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| Abstract: | A simple and sensitive chemiluminescence method for the determination of spermine was developed, based on the spermine-copper (1∶1) unsaturated complex formed by copper and spermine, which had a strang catalytic effect on the chemiluminescence reaction between the luminol and hydrogen peroxide. The detection limit is 4×10~(-9) mol/L for spermine and the linear range extends from 10~(-7) to 10~(-5) mol/L. The relative standard deviation of spermine was 5.55%. |
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| Keywords: | luminol chemiluminescence spermine copper |
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