| 免疫组织化学法定位猪脾脏中脱氧雪腐镰刀菌烯醇的研究 |
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| 引用本文： | 卢培成,贺利民,牛更智,剡海阔,马勇江,范小龙,李玉谷,邓衔柏.免疫组织化学法定位猪脾脏中脱氧雪腐镰刀菌烯醇的研究[J].现代农业科技,2010(18):292-293,296. |
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| 作者姓名： | 卢培成 贺利民 牛更智 剡海阔 马勇江 范小龙 李玉谷 邓衔柏 |
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| 作者单位： | 华南农业大兽医学院,广东广州,510642 |
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| 摘 要： | 利用免疫组织化学法研究了脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON)在猪脾脏分布定位,为DON体内残留及DON对免疫系统影响进一步研究提供依据。选用10头健康阉公猪,随机分为二组,一组经乳静脉注射DON标准品,注射剂量为75μg/kg体重,另一组经乳静脉注射相当体积生理盐水。注射完成后开始计时,并观察记录猪的反应情况,待30min后放血宰杀,并迅速取脾脏,4%多聚甲醛固定,经脱水、包埋及切片后,采用免疫组化法定位脾脏中DON的分布情况。免疫组化法定位结果显示,在75μg/kg体重给药组中,脾组织中的血管壁周围见有DON阳性反应颗粒,而对照组未见阳性反应颗粒。
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| 关 键 词： | 脱氧雪腐镰刀菌烯醇 脾脏 猪 免疫组织化学法 |
The Research of Locating DON in Spleen of Pig with Immunohistochemical |
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| Authors: | LU Pei-cheng HE Li-min NIU Geng-zhi YAN Hai-kuo MA Yong-jiang FAN Xiao-long LI Yu-gu DENG Xian-bo |
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| Institution: | LU Pei-cheng HE Li-min NIU Geng-zhi YAN Hai-kuo MA Yong-jiang FAN Xiao-long LI Yu-gu DENG Xian-bo * (College of Veterinary Medicine,South China Agricultural University,Guangzhou Guangdong 510642) |
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| Abstract: | The distribution and location of deoxynivalenol in spleen of pigs by immunohistochemical was studied, which provided a bisis for the residue of DON in the body and the effect of DON on the immune system. In the research,ten healthy clean pigs were used and divided into two groups randomly. One group were given DON standard by breast vein for the dose 75μg/kg b.w., normal saline was injected at the same dosage in the other group. Counted time after the intravenous administration, observed and noted the response,pigs were killed after 30 minutes, took spleen tissue sample quickly, fixed with 4% paraformaldehyde, dehydrated, embedded and sliced, then the immunohistochemical was used to locate the distribution of DON in spleen. The result of the study showed that DON positive particles were found in the spleen tissue around the blood vessel while in the control group no positive particles were saw. |
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| Keywords: | DON spleen pig immunohistochemical |
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