Efficiency of some molecular markers linked to rhizomania resistance gene (Rz
1
) for marker assisted selection in sugar beet |
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| Authors: | Peyman Norouzi Mohammad Sabzehzari Hasan Zeinali |
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| Institution: | 1. Sugar Beet Seed Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
2. Department of Agronomy and Plant Breeding, Faculty of Agricultural Sciences & Engineering, University of Tehran, Karaj, Iran
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| Abstract: | Sugar beet (Beta vulgaris L.) is one of the two major products supplying sugar (sucroses) in the world. Rhizomania is one of the most destructive diseases of sugar beet world-wide. Holly is the major source of resistance to rhizomania. The objectives of this study were to identify the dominant homozygous genotypes resistant to rhizomania using ZN1 molecular marker, to field evaluate S1 progenies of plants already proved to be containing the marker and also to determine the relationship of this and other SCAR (sequence characterized amplified region) markers with SNP1 (single nucleotide polymorphism) marker associated with the Rz 1 gene. Molecular analysis was carried out on 27 O-type populations (consisting of 13 susceptible and 6 resistant genotypes). Field evaluation and scoring of the phenotypic traits including greenness, growth, uniformity and disease score of 12 O-type populations were carried out on a rhizomania-infested field. The percent agreement of coupling marker ZN1 and repulsion marker ZN8 with disease score was 0.91 and 0.93, respectively. Although all O-types had the Rz 1 resistance gene but the phenotypic differences were observed due to the effect of different genetic backgrounds and modifier genes. Overall, the results showed that the selected markers can be used for marker-assisted selection (MAS) to reduce the time and cost of breeding programs and increase the efficiency of selection. |
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