Protection against malaria by vaccination with sporozoite surface protein 2 plus CS protein. |
| |
| Authors: | S Khusmith Y Charoenvit S Kumar M Sedegah R L Beaudoin S L Hoffman |
| |
| Institution: | Malaria Program, Naval Medical Research Institute, Bethesda, MD 20889. |
| |
| Abstract: | The circumsporozoite (CS) protein has been the target for development of malaria sporozoite vaccines for a decade. However, immunization with subunit vaccines based on the CS protein has never given the complete protection found after immunization with irradiated sporozoites. BALB/c mice immunized with irradiated Plasmodium yoelii sporozoites produced antibodies and cytotoxic T cells against a 140-kilodalton protein, sporozoite surface protein 2 (SSP2). Mice immunized with P815 cells that had been transfected with either SSP2 or CS genes were partially protected, and those immunized with a mixture of SSP2 and CS transfectants were completely protected against malaria. These studies emphasize the importance of vaccine delivery systems in achieving protection and define a multi-antigen sporozoite vaccine. |
| |
| Keywords: | |
|
|
