| 京白梨等品种S 基因型鉴定及新基因S28 和S30 的核苷酸序列分析 |
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| 引用本文： | 张妤艳,吴俊,衡伟,张绍铃.京白梨等品种S 基因型鉴定及新基因S28 和S30 的核苷酸序列分析[J].园艺学报,2006,33(3):496-500. |
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| 作者姓名： | 张妤艳 吴俊 衡伟 张绍铃 |
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| 作者单位： | (南京农业大学园艺学院, 江苏南京210095) |
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| 基金项目： | 国家自然科学基金项目(30270926)，高校博士学科点专项科研基金项目(2001030701)，中国博士后科学基金项目(2005037740)，江苏省博士后科研基金资助项目(2005) |
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| 摘 要： | 根据梨S基因高度保守区C1和C3区, 设计1对引物P1和P2, 对梨品种的基因组DNA进行S基因特异扩增、克隆、测序, 并在GenBank中BLAST比较, 确定S 基因特异性片段, 对京白梨等6个供试自交不亲和品种的S基因型比对结果为: 白梨中的‘库尔勒香梨’为S21 S28 , ‘苹果梨’为S17S19 ; 砂梨中的‘台湾蜜梨’为S11 S22 ; 西洋梨中的‘葫芦梨’为Sa Sb; 秋子梨中的‘京白’为S16 S30 , ‘早梨18’为S4 S28。其中S28和S30为首次登录的新S 基因, 在GenBank的登录号分别为AY562394 (库尔勒香梨) 和AY876945 (京白) 。
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| 关 键 词： | 梨 自交不亲和性 S基因 S基因型鉴定 |
| 文章编号： | 0513-353（2006）03-0496-05 |
| 收稿时间： | 2005-06-26 |
| 修稿时间： | 2005-06-262005-09-06 |
Identification of S-Genotypes of Pear Cultivars and Analyses of Nucleotide Sequences of S28-RNase and S30-RNase |
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| Zhang Yuyan,Wu Jun,Heng Wei,Zhang Shaoling.Identification of S-Genotypes of Pear Cultivars and Analyses of Nucleotide Sequences of S28-RNase and S30-RNase[J].Acta Horticulturae Sinica,2006,33(3):496-500. |
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| Authors: | Zhang Yuyan Wu Jun Heng Wei Zhang Shaoling |
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| Institution: | (College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China) |
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| Abstract: | Partial S-RNase genes of pear were amplified and cloned by using a pair of primers ( P1 and P2) based on the conserved regions C1 and C3 of pear S-RNase structure in six cultivars of unknown S-geontypes. The PCR products were sequenced and compared in GenBank. The S-genotypes of the six tested self-incompatible cultivars were identified as follows: ‘Kuerle Xiangli’ ( Pyrus bretschneideri Rehd. ) was S21 S28 ,‘Pingguoli’ ( P. bretschneideri Rehd. ) was S17S19 , ‘TaiwanMili’ ( P. pyrifolia Nakai) was S11 S22 , ‘Hululi’ ( P. comm unis L. ) was Sa Sb , ‘J ingbai’ ( P. ussriensis Max. ) was S16 S30 , ‘Zaoli 18’ ( P. ussriensis Max. ) was S4 S28. S28-RNase and S30-RNase were deposited in GenBank under the accession numbers of AY562394 and AY876945. |
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| Keywords: | Pyrus Self-incompatibility S-RNase gene S-genotyping |
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