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Investigation of Bartonella henselae in cats in Ankara, Turkey
Authors:Celebi B  Kilic S  Aydin N  Tarhan G  Carhan A  Babur C
Institution:Communicable Diseases Research Department, Refik Saydam National Hygiene Center, Ankara, Turkey.
Abstract:The purpose of this study was to determine Bartonella henselae prevalance in cats in Ankara. Whole bloods and sera collected from 256 cats were investigated for the presence feline Bartonella species by culture and sera were tested for the presence of antibodies against B. henselae IgG using immunofluorescence assay. Bartonella species were isolated by blood culture from 24 (9.4%) cats. Bartonella isolates were subjected to restriction fragment length polymorphism (RFLP) by using TaqI and HhaI endonucleases to identify species. Twenty-one isolates were determined as B. henselae and three of 24 isolates were determined as Bartonella clarridgeiae with RFLP. The bacteraemia prevalence and seroprevalence of B. henselae IgG antibodies in cats was detected as 8.2% and 18.6% respectively. This is the first report on B. henselea and B. clarridgeiae in cats in Turkey.
Keywords:Cat  Bartonella henselae  culture  serology  PCR  Turkey
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