| 油菜花叶病毒武汉株系基因组全序列分析 |
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| 引用本文： | 晏立英,XU Ze-yong,陈坤荣.油菜花叶病毒武汉株系基因组全序列分析[J].植物病理学报,2008,38(4):394-400. |
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| 作者姓名： | 晏立英 XU Ze-yong 陈坤荣 |
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| 作者单位： | 中国农业科学院油料作物研究所油料作物遗传改良农业部重点实验室, 武汉 430062 |
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| 摘 要： | 油菜花叶病毒(Oilseed rape mosaic virus,ORMV)武汉株系(Wh)基因组全序列分析表明,该株系基因组全长6301nt,与烟草花叶病毒属(Tobamovirus)亚组Ⅲ病毒基因组结构相似,含4个开放阅读框架(open reading frame,ORF),ORF2与ORF3有77nt的重叠区。与该属其他病毒对应ORF的核苷酸及编码蛋白氨基酸序列比对,ORMV-Wh与亚组Ⅲ的ORMV、车前草花叶病毒上海株系(Ribgrass mosaic virus,RMV-Sh)、车前草花叶病毒凤仙花株系(RMV-Imp)和烟草花叶病毒十字花科和大蒜株系(Tobacco mosaic virus,TMV-Cg)一致性最高,均超过95%。4种蛋白的氨基酸序列系统进化树构建将Tobamovirus亚组Ⅲ株系分为3个群,ORMV-Wh归为ORMV代表的株系群。
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| 关 键 词： | 油菜花叶病毒 武汉株系 序列分析 |
Full sequence analysis of genomic RNAs of Oilseed rape mosaic virus Wh isolate |
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| YAN Li-ying,XU Ze-yong,CHEN Kun-rong.Full sequence analysis of genomic RNAs of Oilseed rape mosaic virus Wh isolate[J].Acta Phytopathologica Sinica,2008,38(4):394-400. |
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| Authors: | YAN Li-ying XU Ze-yong CHEN Kun-rong |
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| Institution: | Key Laboratory of Genetic Improvement for Oil Crops, Ministry of Agriculture, Oil Crop Research Institute of Chinese Academy of Agricultural Sciences, Wuhan 430062, China |
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| Abstract: | The complete nucleotide sequence of Wuhan isolate of Oilseed rape mosaic virus (ORMV-Wh) was determined. ORMV-Wh genome was 6 301 nucleotides long, and its organization was similar to those of other strains of crucifer tobamoviruses. Sequence comparison of ORMV-Wh with the corresponding region of other strains of crucifer tobamoviruses revealed the highest identity, over 95 percent with Oilseed rape mosaic virus (ORMV), Ribgrass mosaic virus Shanghai strain (RMV-Sh), Ribgrass mosaic virus impatient strain (RMV-Imp) and Tobacco mosaic virus crucifer and garlic strain (TMV-Cg). Phylogentic trees of amino acid sequence of 4 proteins revealed that there were three clusters in Tobamovirus subgroup Ⅲ, and ORMV-Wh was belonged to the ORMV cluster. |
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| Keywords: | Oilseed rape mosaic virus Wuhan strain sequence analysis |
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