| 刚毛柽柳富含甘氨酸RNA结合蛋白ThGRP1基因克隆与表达分析 |
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| 引用本文： | 姜波,高彩球,王玉成,于丽丽,杨传平.刚毛柽柳富含甘氨酸RNA结合蛋白ThGRP1基因克隆与表达分析[J].林业科学研究,2011,24(2):256-262. |
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| 作者姓名： | 姜波 高彩球 王玉成 于丽丽 杨传平 |
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| 作者单位： | 东北林业大学林学院,林木遗传育种与生物技术教育部重点实验室,黑龙江,哈尔滨,150040 |
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| 基金项目： | 国家自然科学基金面上项目(30972386);东北林业大学研究生科技创新项目(000-41110710) |
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| 摘 要： | 在有机体的生长和发育过程中,基因表达在转录、转录后和翻译水平都受到严格地调控。转录调控是基因表达调控的第一步,曾被认为是基因表达的主要调控机制,但是随着对转录后调控机制越来
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| 关 键 词： | 刚毛柽柳 富含甘氨酸RNA结合蛋白 基因克隆 实时定量RT-PCR |
| 收稿时间： | 2010/7/29 0:00:00 |
Cloning and Expression Analysis of A Glycine-rich RNA-binding Protein Gene from Tamarix hispida |
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| JIANG Bo,GAO Cai-qiu,WANG Yu-cheng,YU Li-li and YANG Chuan-ping.Cloning and Expression Analysis of A Glycine-rich RNA-binding Protein Gene from Tamarix hispida[J].Forest Research,2011,24(2):256-262. |
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| Authors: | JIANG Bo GAO Cai-qiu WANG Yu-cheng YU Li-li and YANG Chuan-ping |
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| Institution: | Key Laboratory of Forest Tree Genetic improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China;Key Laboratory of Forest Tree Genetic improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China;Key Laboratory of Forest Tree Genetic improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China;Key Laboratory of Forest Tree Genetic improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China;Key Laboratory of Forest Tree Genetic improvement and Biotechnology of Ministry of Education, Northeast Forestry University, Harbin 150040, Heilongjiang, China |
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| Abstract: | The full-length cDNA of a presumed glycine-rich ribonucleic acid (RNA) binding protein (ThGRP1) gene was isolated from a cDNA library of Tamarix hispida leaves. The ThGRP1 gene with 800 bp in length contains 432-base pair (bp) open reading frame (ORF) and encodes a 143-amino-acid polypeptide with a predicted molecular weight of 14.95 kDa and pI of 5.36. Expression of ThGRP1 in roots, stems and leaves of T. hispida with PEG, NaCl, NaHCO3 or CdCl2 treatments was studied using real-time PCR. The results showed that ThGRP1 can be induced by these stresses. Especially, the expression of ThGRP1 in roots of T. hispida was induced under all stresses at 6, 12, 24, 48 and 72 h. |
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| Keywords: | Tamarix hispida ThGRP1 gene clone real time RT-PCR |
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