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| 运输温度与时间对猪孤雌胚胎发育的影响 | |
| 引用本文： | 张卫红,闫军,冯冲,龙川,张兆旺,潘登科.运输温度与时间对猪孤雌胚胎发育的影响[J].安徽农业科学,2009,37(13):5998-5999. |
| 作者姓名： | 张卫红 闫军 冯冲 龙川 张兆旺 潘登科 |
| 作者单位： | 1. 甘肃农业大学动物科学技术学院,甘肃兰州,730070;中国农业科学院北京畜牧兽医研究所,畜禽遗传资源与利用农业部重点实验室,北京,100193 2. 中国农业科学院北京畜牧兽医研究所,畜禽遗传资源与利用农业部重点实验室,北京,100193 3. 甘肃农业大学动物科学技术学院,甘肃兰州,730070 |
| 基金项目： | 国家“863”项目(2006AA02Z113); 国家基本科研业务费(ywf-td-1); 中国农业科学院院长基金资助 |
| 摘 要： | 目的]比较不同运输温度与时间对猪孤雌胚胎发育的影响，确立猪克隆胚胎的最佳运输温度与时间。方法]研究不同温度（37、38、39℃）对孤雌胚胎卵裂率、囊胚率的影响；不同时间（2、3、4h）对孤雌胚胎卵裂率、囊胚率的影响。结果]培养3h后，在39℃运输时，孤雌胚胎的卵裂率和囊胚率与37℃、38℃的卵裂率和囊胚率相比，差异显著（P〈0．05）；培养3h后，孤雌胚胎的卵裂率和囊胚率与培养2h、4h的卵裂率和囊胚率相比，差异显著（P〈0．05）。结论]在39℃，3h内进行孤雌胚胎的运输，能获得较高的卵裂率、囊胚率。 |
| 关 键 词： | 猪 运输时间 运输温度 孤雌胚胎 卵裂率 囊胚率 |
Effects of Different Transport Temperature and Transport Time on Development of Porcine Parthenogenetic Embryo |
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| ZHANG Wei-hong et al.Effects of Different Transport Temperature and Transport Time on Development of Porcine Parthenogenetic Embryo[J].Journal of Anhui Agricultural Sciences,2009,37(13):5998-5999. | |
| Authors: | ZHANG Wei-hong |
| Institution: | ZHANG Wei-hong et al (College of Animal Science , Technology,Gansu Agricultural University,Lanzhou,Gansu 730070) |
| Abstract: | Objective]The research aimed to establish the best transport temperature and transport time of porcine parthenogenetic ernbryo clone.Method]The effects of different temperatures(37,38,39 ℃) on parthenogenetic embryo cleavage and blastocyst rate and the effects of different time(2,3,4 h) on parthenogenetic embryo cleavage and blastocyst rate were studied.Result]There were statistical differences at 0.05 level of the parthenogenetic cleavage rate and blastocyst rate between development of parthenogenetic embryos at 39,37 and 38 ℃.And there were statistical differences at 0.05 level of the parthenogenetic cleavage rate and blastocyst rate between development of parthenogenetic embryos in 3,2 and 4 h.Conclusion]This study suggested that carrying out the transport of parthenogenetic embryos at 39 ℃,within 3 h could obtain a higher cleavage rate and blastocyst rate. |
| Keywords: | Porcine Transportation time Transportation temperature Parthenogenetic embryos Cleavage rate Blastocyst rate |
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