| 鸡新城疫病毒F48E9强毒株NP基因的序列分析 |
| |
| 引用本文： | 陈珍,施少华,程龙飞,傅光华,陈红梅,万春和,林芳,林建生,黄瑜.鸡新城疫病毒F48E9强毒株NP基因的序列分析[J].福建农业学报,2009,24(5):385-389. |
| |
| 作者姓名： | 陈珍 施少华 程龙飞 傅光华 陈红梅 万春和 林芳 林建生 黄瑜 |
| |
| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福建,福州,350013
2. 福建省农业科学院畜牧兽医研究所,福建,福州,350013;福建省畜禽疫病防治工程技术研究中心,福建,福州,350013 |
| |
| 基金项目： | 国家自然科学基金资助项目，国家"973"子项目 |
| |
| 摘 要： | 根据GenBank登录的鸡新城疫病毒NP基因序列设计引物，利用RT—PCR对F48E9强毒株NP基因进行扩增，将扩增产物经纯化后连入pMD18-T载体，通过PCR和酶切鉴定出阳性重组质粒，经测序后获得F48E9 NP基因序列。经分析发现该基因的ORF总长为1470bp，编码489aa，NP蛋白有4个高度保守的Cys位点，分别位于第55、78、139、213位，且在401～440位和461～481位区域变异较大。将F48E9株与19株参考毒株的相应序列进行比较得到核苷酸序列同源性为87．9％～92．3％，氨基酸序列同源性较高，为92．2％～98．0％，遗传进化分析表明F48E9株相对独立，与参考毒株的遗传距离较远。
|
| 关 键 词： | 鸡新城疫病毒 F48E9株 NP基因 序列分析 |
Sequence analysis on nucleocapsid protein gene of velogenic chicken-origin Newcastle disease virus,F48E9 strain |
| |
| CHEN Zhen,SHI Shao-hua,CHENG Long-fei,FU Guang-hua,Chen Hong-mei,WAN Chun-he,LIN Fang,LIN Jian-sheng,HUANG Yu.Sequence analysis on nucleocapsid protein gene of velogenic chicken-origin Newcastle disease virus,F48E9 strain[J].Fujian Journal of Agricultural Sciences,2009,24(5):385-389. |
| |
| Authors: | CHEN Zhen SHI Shao-hua CHENG Long-fei FU Guang-hua Chen Hong-mei WAN Chun-he LIN Fang LIN Jian-sheng HUANG Yu |
| |
| Institution: | CHEN Zhen~1,SHI Shao-hua~(1,2),CHENG Long-fei~(1,FU Guang-hua~(1,Chen Hong-mei~1,WAN Chun-he~1,LIN Fang~1,LIN Jian-sheng~1,HUANG Yu~(1,2) (1.Institute of Animal Husbandry and Veterinary,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China,2.Fujian Animal Diseases Control Techology Development Center,China) |
| |
| Abstract: | The nucleocapsid protein gene of velogenic,chicken-origin,Newcastle disease virus(NDV) F48E9 was amplified by RT-PCR based on a specific pair of primers designed according to the chicken-origin NDVs NP gene sequences published at GenBank.The purified RT-PCR products were cloned onto the pMD18-T vectors,and then,the positive recombinants had been identified by PCR and enzyme digestion.The sequencing results indicated that the F48E9 NP gene had an opening read frame of 1470nt in length with a coded polypeptid... |
| |
| Keywords: | Newcastle disease virus F48E9 strain nucleocapsid protein gene cloning |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
