| 板栗疫病菌脱毒方法的比较研究 |
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| 引用本文： | 康宁,蓝秀万,陈保善.板栗疫病菌脱毒方法的比较研究[J].植物病理学报,2007,37(1):83-87. |
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| 作者姓名： | 康宁 蓝秀万 陈保善 |
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| 作者单位： | 广西亚热带生物资源保护利用重点实验室, 微生物及植物遗传工程教育部重点实验室, 广西大学生命科学与技术学院, 南宁 530005 |
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| 基金项目： | 国家自然科学基金;国家自然科学基金;广西自然科学基金;广西新世纪十百千人才工程基金 |
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| 摘 要： | 病毒与宿主相互作用中,存在宿主主动或被动摆脱病毒的机制。本研究用光照诱导-单孢分离、原生质体再生和菌丝尖端分离3种方法对携带低毒病毒的板栗疫病菌(Cryphonectria parasitica)的EP721、EP713和Euro7三个菌株的脱毒效率进行了比较。结果表明:经光照诱导-单孢分离后所有菌株均可获得脱毒菌株,而原生质体再生对EP721的脱毒效率最高,是继光照诱导-单孢分离脱毒法之后另一种新的有效的脱毒方法,特别适用于不产孢的真菌脱毒。
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| 关 键 词： | 板栗疫病菌 脱毒 原生质体再生 |
| 文章编号： | 0412-0914(2007)01-0083-05 |
| 修稿时间： | 2005年11月21 |
Comparison of methods to eliminate virus from chestnut blight fungus Cryphonectria parasitica |
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| KANG Ning,LAN Xiu-wan,CHEN Bao-shan.Comparison of methods to eliminate virus from chestnut blight fungus Cryphonectria parasitica[J].Acta Phytopathologica Sinica,2007,37(1):83-87. |
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| Authors: | KANG Ning LAN Xiu-wan CHEN Bao-shan |
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| Institution: | Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, The Key Laboratory of Ministry of Education of China for Microbial and Plant Genetic Engineering, College of Life Science and Technology, Guangxi University, Nanning 530005, China |
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| Abstract: | In virus-host interaction,there are mechanisms for a host to eliminate virus either actively or passively.Efficiency of light induced sporulation-single conidial spore isolation,spheroplast regeneration,and meristemic culture in eliminating or escaping virus from the hypovirus-infected Cryphonectria parasitica strains EP721,EP713 and Euro7 was evaluated.Single spore isolation was effective in all treatments though with va-ried efficiency and spheroplast regeneration was also an efficient method to obtain virus-free colonies from infected strains.The latter may be particularly useful in obtaining the virus-free culture in case where a virus-infected fungus does not produce spores. |
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| Keywords: | Cryphonectria parasitica virus elimination spheroplast regeneration |
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